Document Detail


Growth inhibition and induction of early apoptosis by arenicolsterol A, a novel cytotoxic enolic sulphated sterol from the marine annelid, Arenicola cristata.
MedLine Citation:
PMID:  17994393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arenicolsterol A (ASA), a novel cytotoxic enolic sulphated sterol, was isolated from the marine annelid, Arenicola cristata (AC). Growth inhibition of this compound on cancer cell lines was determined by MTT assay and suppression of tumour stem cells colony formation. The results showed that ASA was selectively cytotoxic on HeLa cell line (IC(50) = 6.00 +/- 1.16 micromol L(- 1) on HeLa cell line, IC(50) = 10.85 +/- 0.97 micromol L(- 1) on 929 cell line and 14.72 +/- 1.55 micromol L(- 1) on NCI-h6 cell line). In addition, the apoptosis induced by ASA was verified from monitoring the stainability with Annexin V and propidium iodine by a fluorescence-activated cell sorter. The experimental data confirmed that ASA could induce apoptosis in HeLa cells by arresting early stage in apoptosis. Meanwhile, the apoptosis was found to be correlative with the inhibition of the protein tyrosine phosphatases (cdc25A, cdc25B, JSP1, etc). Therefore, ASA might be a novel promising precursor of anticancer medicines.
Authors:
Liang Wang; Bin Chen; Xian-Rong Shen; Yue-Yang Zhou; Ding-Wen Jiang; Jia Li; Ji-Lie Kong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of Asian natural products research     Volume:  9     ISSN:  1028-6020     ISO Abbreviation:  J Asian Nat Prod Res     Publication Date:    2007 Sep-Dec
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-05-14     Revised Date:  2009-07-21    
Medline Journal Info:
Nlm Unique ID:  100888334     Medline TA:  J Asian Nat Prod Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  753-61     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Fudan University, Shanghai, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Annelida / chemistry*
Apoptosis / drug effects*
Cell Division / drug effects*
Cell Line
Cell Line, Tumor
Drug Screening Assays, Antitumor
Humans
Marine Biology
Mice
Molecular Structure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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