Document Detail


Growth inhibition and induction of apoptosis in human oral squamous cell carcinoma Tca-8113 cell lines by Shikonin was partly through the inactivation of NF-kappaB pathway.
MedLine Citation:
PMID:  18167055     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shikonin, a naphthoquinone pigment isolated from the Chinese herbal therapeutic, Zicao, has been shown to exhibit antioxidant and anticancer effects. In this study, its ability to induce apoptosis in cultured Tca-8113 oral cancer cells was studied. Treatment of the Tca-8113 cells with a variety of concentrations of Shikonin (10-40 microm) resulted in dose- and time-dependent sequences of events marked by apoptosis, as shown by the loss of cell viability, chromatin condensation, internucleosomal DNA fragmentation and sub-G1 phase accumulation. Furthermore, apoptosis in the Tca-8113 cells was accompanied by the activation of protease caspase-8, -9, -3 and low expression of Bcl-2 protein. Interestingly, inactivation of the NF-kappaB pathway was found in shikonin-induced apoptosis in Tca-8113 cells. These results raise the possibility that the anti-tumor effects of Shikonin in Tca-8113 cells are at least partly through the inactivation of the NF-kappaB pathway and subsequent activation of protease caspase family. Pharmacological inhibition of the NF-kappaB activity by Shikonin might be a powerful treatment option for OSCC in which activation of NF-kappaB plays a critical role in tumor growth and progression.
Authors:
Ruan Min; Ji Tong; Yang Wenjun; Duan Wenhu; Zhou Xiaojian; He Jiacai; Zhou Jian; Chen Wantao; Zhang Chenping
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  22     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  407-15     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Affiliated No. 9 People's Hospital, College of Medicine, Shanghai Jiao Tong University, Shanghai 200011, PRC.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Antibodies, Monoclonal / metabolism
Antineoplastic Agents, Phytogenic / pharmacology*
Apoptosis / drug effects*
Carcinoma, Squamous Cell / pathology
Caspases / analysis,  metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
DNA / analysis
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / pharmacology*
Gene Expression / drug effects
Humans
NF-kappa B / drug effects*,  metabolism
Naphthoquinones / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / analysis,  biosynthesis
Time Factors
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Antibodies, Monoclonal; 0/Antineoplastic Agents, Phytogenic; 0/Drugs, Chinese Herbal; 0/NF-kappa B; 0/Naphthoquinones; 0/Proto-Oncogene Proteins c-bcl-2; 54952-43-1/shikonin; 9007-49-2/DNA; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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