| Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. | |
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MedLine Citation:
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PMID: 16399223 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antrodia camphorata (A. camphorata) is well known in Taiwan as a traditional Chinese medicine, and it has been shown to exhibit antioxidant and anticancer effects. In this study, therefore, its ability to induce apoptosis in cultured MCF-7 breast cancer cells was studied. Treatment of the MCF-7 cells with a variety of concentrations of the fermented culture broth of A. camphorata (25-150 microg/ml) resulted in dose- and time-dependent sequences of events marked by apoptosis, as shown by loss of cell viability, chromatin condensation, internucleosomal DNA fragmentation, and sub-G1 phase accumulation. Furthermore, apoptosis in the MCF-7 cells was accompanied by the release of cytochrome c, activation of caspase 3, and specific proteolytic cleavage of poly (ADP-ribose) polymerase (PARP). Although, the A. camphorata-induced apoptosis was associated with Bax protein levels, negligible Bcl-2 reduction was observed. Interestingly, A. camphorata induced dose-dependent reactive oxygen species (ROS) generation in MCF-7 cells. Analysis of the data suggests that A. camphorata exerts antiproliferative action and growth inhibition on MCF-7 cells through apoptosis induction, and that it may have anticancer properties valuable for application in drug products. |
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Authors:
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Hsin-Ling Yang; Chee-Shan Chen; Wen-Huei Chang; Fung-Jou Lu; Yu-Ching Lai; Chin-Chu Chen; Tzong-Hsiung Hseu; Chiung-Tsun Kuo; You-Cheng Hseu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer letters Volume: 231 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-09 Completed Date: 2006-03-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 215-27 Citation Subset: IM |
Affiliation:
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Institute of Nutrition, China Medical University, Taichung, Taiwan, ROC. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Annexin A5
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metabolism Apoptosis / drug effects* Breast Neoplasms / drug therapy*, pathology Caspases / metabolism Cell Proliferation / drug effects Cytochromes c / metabolism Dose-Response Relationship, Drug Drugs, Chinese Herbal / therapeutic use* Female Flow Cytometry G1 Phase / drug effects* Humans Poly(ADP-ribose) Polymerases / metabolism Polyporales / chemistry* Proto-Oncogene Proteins c-bcl-2 / metabolism Reactive Oxygen Species / metabolism* Tumor Cells, Cultured bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Annexin A5; 0/Drugs, Chinese Herbal; 0/Proto-Oncogene Proteins c-bcl-2; 0/Reactive Oxygen Species; 0/bcl-2-Associated X Protein; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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