| Growth inhibition in animal cell culture. The effect of lactate and ammonia. | |
| | |
MedLine Citation:
|
PMID: 1952924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Eight independent cell lines accumulated ammonia in culture to concentrations between 1.3 and 2.9 mM. The growth inhibition of such concentrations of ammonium chloride when added to culture medium was variable. The cell lines tested could be divided into 3 groups depending on their growth response to 2 mM added NH4Cl. In the first group (293, HDF, Vero, and PQXB1/2) little (less than 14%) or no growth inhibition occurred. In the second group (McCoy and MDCK) a reduction in final cell yield of 50-60% was observed. The third group (HeLa and BHK) was most sensitive to the effects of NH4Cl with growth inhibition (greater than 75%) compared to controls. The growth inhibitory effect of added lactate up to 20 mM was negligible (less than 10%) for 3 cell lines, although one cell line (PQXB1/2) showed greater sensitivity. The interactive effects of ammonia and lactate were determined in a matrix experiment. At lactate (greater than 12 mM) and ammonia (1-4 mM), the growth inhibitory effects of the two components were synergistic. However, at low concentrations of lactate (less than 12 mM) the toxic effect of ammonia was reduced. A proposed mechanism for the sparing effect of lactate on ammonia toxicity is discussed. This may have importance in developing strategies for the optimal growth of ammonia-sensitive cell lines. |
| | |
Authors:
|
T Hassell; S Gleave; M Butler |
Related Documents
:
|
6800304 - Regulation of beta-d-galactosidase synthesis in candida pseudotropicalis. 8070614 - Effects of glucose on insulin secretion, glucokinase activity, and transgene expression... 2546844 - Effect of galactose and glucose levels and sorbinil treatment on myo-inositol metabolis... 21348624 - Evaluation of five antischizophrenic agents against toxoplasma gondii in human cell cul... 18207284 - Survival of clostridium difficile on copper and steel: futuristic options for hospital ... 17943274 - Arachidonic acid pathway activates multidrug resistance related protein in cultured hum... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: 30 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 1991 Jul |
Date Detail:
|
Created Date: 1991-12-06 Completed Date: 1991-12-06 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 29-41 Citation Subset: IM |
Affiliation:
|
Department of Biological Sciences, Manchester Polytechnic, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Ammonia
/
metabolism,
pharmacology* Ammonium Chloride / pharmacology Animals Cell Division / drug effects* Cell Line Cricetinae Dogs Drug Synergism Humans Lactates / metabolism, pharmacology* Lactic Acid Mice |
| Chemical | |
Reg. No./Substance:
|
0/Lactates; 12125-02-9/Ammonium Chloride; 50-21-5/Lactic Acid; 7664-41-7/Ammonia |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rapid and simple isolation of multigram goat IgG from serum using Avid AL and radial flow column.
Next Document: Immobilization of binding proteins on nonporous supports. Comparison of protein loading, activity, a...