Document Detail

Growth inhibition and augmentation of mouse myeloid leukemic cell line differentiation by interleukin 1.
MedLine Citation:
PMID:  3494508     Owner:  NLM     Status:  MEDLINE    
The effect of interleukin 1 (IL 1) on the growth and differentiation of mouse myeloid leukemic cell line (M1) cells into macrophages has been studied. Purified human IL 1 beta appeared to be growth inhibitory (maximum, 50%) for M1 cells based on cell counts and [3H]thymidine incorporation. The replication of M1 cells was also inhibited by lipopolysaccharide (LPS), and as little as 1 unit/ml IL 1 augmented the growth inhibition by LPS. Although IL 1 inhibited M1 cell growth, it did not induce cell differentiation by the criteria of either effect on expression of Fc receptors or on phagocytic ability. However, IL 1 augmented M1 cell differentiation in conjunction with LPS. At low doses of LPS, addition of IL 1 induced differentiation even though LPS and IL 1 by themselves did not induce differentiation. Cells treated with IL 1 for 1 day and then with LPS for an additional 2 days showed considerable augmentation of Fc receptor expression, while cells treated with the same stimuli in the reverse sequence exhibited only a low level of differentiation. Cells treated with medium alone followed by LPS showed moderate increase in Fc receptor expression. In addition, exposure of cells to IL 1 for at least 16 h was required for IL 1 augmenting effect. Therefore, IL 1 appeared to primarily influence M1 cells to become more sensitive to LPS. Treatment with both of IL 1 and LPS induced differentiation of a LPS-resistant clone of M1 cells, and IL 1 pretreatment rendered the resistant clone to become responsive to the differentiation inducing effect of LPS. Culture supernatants of M1 cells after stimulation with LPS contained IL 1-like activity by thymocyte comitogenic assays. In addition, mouse recombinant IL 1 alpha appeared to have the same activity as purified human IL 1 beta on the growth and differentiation of M1 cells. These results suggest that IL 1 may play an important role in mouse myeloid leukemic cell differentiation by acting as an autostimulating factor. IL 1 has been shown to be growth inhibitory and cytocidal for several tumor cell lines. Our results therefore suggest that the effects of IL 1 may result in the induction of terminal differentiation of some tumor cells.
K Onozaki; T Tamatani; T Hashimoto; K Matsushima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  47     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1987 May 
Date Detail:
Created Date:  1987-05-27     Completed Date:  1987-05-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2397-402     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation / drug effects
Cell Division / drug effects
Interleukin-1 / pharmacology*
Leukemia, Myeloid, Acute / pathology*
Lipopolysaccharides / pharmacology
Macrophages / cytology,  immunology
Phagocytosis / drug effects
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Interleukin-1; 0/Lipopolysaccharides; 0/Recombinant Proteins

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