Document Detail


Growth inhibition of Monodus subterraneus by free fatty acids.
MedLine Citation:
PMID:  18546417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monodus subterraneus is a microalga, which is known for its high eicosapentaenoic acid (EPA; 20:5omega3) content. To produce EPA commercially, high volumetric productivities of microalgae are required. These high productivities can be reached in flat panel photobioreactors with small optical paths that have to be operated at high cell densities (>10 g/L). However, at these cell densities a reduction of productivity is observed. This growth inhibition is probably caused by growth inhibitors released by the microalgae, which have been suggested to be fatty acids. Our aim was to investigate if free fatty acids produced by M. subterraneus inhibited growth of this species. Therefore a bioassay was developed and saturated, unsaturated and poly-unsaturated fatty acids occurring in Monodus were tested on their growth inhibiting properties. Growth of M. subterraneus was completely inhibited at a saturated concentration (96 microM) of palmitoleic acid (16:1omega7). But, the saturated fatty acid palmitic acid (16:0) and the mono-saturated oleic acid (18:1omega9) were much stronger inhibitors. Growth was inhibited for 50% already at concentrations of 0.4 microM 16:0 and 3 microM 18:1omega9, respectively. These fatty acids probably cause the growth inhibition in high cell density cultures of M. subterraneus.
Authors:
R Bosma; K Miazek; S M Willemsen; M H Vermuë; R H Wijffels
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  101     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1108-14     Citation Subset:  IM    
Affiliation:
Department of Agrotechnology and Food Sciences, Bioprocess Engineering Group, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. rouke.bosma@wur.nl
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MeSH Terms
Descriptor/Qualifier:
Algae / drug effects*,  growth & development*,  metabolism
Biomass
Fatty Acids, Nonesterified / pharmacology*,  secretion
Fatty Acids, Unsaturated / pharmacology
Palmitic Acids / metabolism,  pharmacology
Photobiology
Refractometry
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 0/Fatty Acids, Unsaturated; 0/Palmitic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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