| Growth-inhibiting and apoptosis-inducing effects of Tanshinone II A on human gastric carcinoma cells. | |
| | |
MedLine Citation:
|
PMID: 18231749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To explore the effects of Tanshinone II A on the proliferation, apoptosis and gene expression of p53 and bcl-2 in human gastric carcinoma MKN-45 cells. Cell count and MTT assay were used to study the proliferation-inhibiting effect of Tanshinone II A on MKN-45 cells. The effect of Tanshinone II A on the cell cycle and apoptosis of MKN-45 cells were examined by propidium iodide (PI) staining and flow cytometry. Semi-quantitative RT-PCR was used to further verify the expression of p53 and bcl-2 gene after exposure to Tanshinone A in MKN-45 cells. The results showed that Tanshinone A significantly inhibited the growth and proliferation of MKN-45 cells in a dose-and time-dependent manner (P<0.05). Tanshinone A arrested MKN-45 cells in G(2)/M phase which led to an obvious accumulation of G(2)/M phase cells while decreased number of G(0)/G(1) phase cells. This resulted in apoptosis of MKN-45 cells and the apoptosis rate was as high as 43.91% after treatment with 2.0 microg/mL Tanshinone II A for 96 h. It was also found that Tanshinone II A up-regulated expression of p53 gene and down-regulated expression of bcl-2 gene. The cytostatic and antiproliferative effect of Tanshinone II A makes it a promising anticancer agent for the treatment of gastric carcinoma. |
| | |
Authors:
|
Xiarong Dong; Jihua Dong; Gang Peng |
Related Documents
:
|
17786719 - Guanosine acts intracellularly to initiate apoptosis in nb4 cells: a role for nucleosid... 11744329 - Effect of overexpression of bcl-2 on cellular oxidative damage, nitric oxide production... 21036719 - Sodium butyrate with ucn-01 has marked antitumour activity against cervical cancer cells. 11239649 - Aspirin effects on endometrial cancer cell growth. 16541129 - Bcl-2 down modulation in wehi-3b/ctres cells resistant to cholera toxin (ct)-induced ap... 10978339 - Regulation of apoptosis and cell cycle progression by mcl1. differential role of prolif... 15341649 - Newly secreted adenylate cyclase toxin is responsible for intoxication of target cells ... 16387649 - Coherence and timing of cell cycle start examined at single-cell resolution. 2841579 - Physical monitoring of mating type switching in saccharomyces cerevisiae. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban Volume: 27 ISSN: 1672-0733 ISO Abbreviation: J. Huazhong Univ. Sci. Technol. Med. Sci. Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2008-01-30 Completed Date: 2010-12-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101169627 Medline TA: J Huazhong Univ Sci Technolog Med Sci Country: China |
Other Details:
|
Languages: eng Pagination: 706-9 Citation Subset: IM |
Affiliation:
|
Cancer Center, Huazhong University of Science and Technology, Wuhan, 430022, China. xr.dong@gmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antineoplastic Agents, Phytogenic
/
pharmacology* Apoptosis / drug effects* Cell Line, Tumor Cell Proliferation / drug effects Drugs, Chinese Herbal / pharmacology Gene Expression Regulation, Neoplastic Humans Phenanthrenes / pharmacology* Proto-Oncogene Proteins c-bcl-2 / metabolism Stomach Neoplasms / pathology* Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents, Phytogenic; 0/Drugs, Chinese Herbal; 0/Phenanthrenes; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 568-73-0/tanshinone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical value of contrast-enhanced ultrasound in differentiating benign and malignant focal liver l...
Next Document: Determination of carboplatin dose by area under the curve in combination chemotherapy for senile non...