Document Detail


Growth of human renal cortical tissue on collagen gel.
MedLine Citation:
PMID:  1371503     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A model system for 3-dimensional "native-state" culture of tissues on collagen gels (Proc. Natl. Acad. Sci. USA 86:2013-2017; 1989) has been applied in this study to histologically normal human renal cortical tissue from 11 patients undergoing nephrectomy for renal cell carcinoma elsewhere in the kidney. Microbial contamination occurred in 12/90 cultures, the rest (78) were studied by visual inspection, histology, immunohistochemical analysis for pankeratin (epithelial cell origin), vimentin (mesenchymal cell origin), and p-glycoprotein (associated with proximal tubules), transmission electron microscopy (EM), incorporation of tritiated thymidine (3HTdR). In the first 10 days, explants showed 3HTdR-labeled cells in tubule structures. The surrounding gel was invaded by cells forming tubule structures, sometimes with basement membrane. Some of these cells showed labeling by 3HTdR and immunostaining positive for pankeratin and p-glycoprotein. EM showed well-polarized epithelial cells in tubule structures with tight junctions, interdigitating lateral processes, and microvilli characteristic of proximal and distal convoluted tubules. 3HTdR-labeled cells in tubule structures were observed even 2 mo. after Passage 1, 6 mo. after the initial explantation. Tubule growth was most active and fibroblast proliferation was negligible from 2 to 4 wk postexplantation. The proliferation of tubulelike cells and formation of tubulelike structures in this system represents an opportunity to study human renal cortical tissue in vitro, under conditions more closely resembling in vivo circumstances than are present in other in vitro systems suitable for long-term study. This model has potential use for in vitro toxicology studies and studies of renal physiology.
Authors:
S G Chang; K Tóth; J D Black; H K Slocum; S D Perrapato; R P Huben; Y M Rustum
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  28A     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-04-02     Completed Date:  1992-04-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  128-35     Citation Subset:  IM    
Affiliation:
Grace Cancer Drug Center and Experimental Therapeutics, Roswell Park Cancer Institute, Buffalo, New York 14263.
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cells, Cultured
Collagen
DNA / biosynthesis
Epithelial Cells
Epithelium / metabolism
Gels
Humans
Keratins / metabolism
Kidney Cortex / cytology*,  metabolism
Membrane Glycoproteins / metabolism
Microscopy, Electron
P-Glycoprotein
Time Factors
Vimentin / metabolism
Grant Support
ID/Acronym/Agency:
CA-16056/CA/NCI NIH HHS; CA-21071/CA/NCI NIH HHS; CA-49816/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Gels; 0/Membrane Glycoproteins; 0/P-Glycoprotein; 0/Vimentin; 68238-35-7/Keratins; 9007-34-5/Collagen; 9007-49-2/DNA

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