Document Detail

Growth of human diploid fibroblasts in the absence of glucose utilization.
MedLine Citation:
PMID:  1069299     Owner:  NLM     Status:  MEDLINE    
Normal human diploid fibroblasts were able to undergo one to two cell divisions without glucose utilization in Eagle's minimum essential medium plus 10% dialyzed fetal calf serum if the medium was supplemented with hypoxanthine, thymidine, and uridine (supplemented medium termed HTU-MEM). Under these conditions, the added purine and pyrimidines were required for nucleic acid synthesis, as shown by the inability of Lesch-Nyhan fibroblasts to grow in HTU-MEM. Normal human diploid fibroblasts continued to produce lactate in HTU-MEM, but at a greatly reduced rate. Since cells grew in HTU-MEM without glucose utilization, the probable energy and carbon source was glutamine, which is present in relatively high concentration. Furthermore, the rate of glutamine utilization per cell division was 2-fold greater in HTU-MEM than in medium with 5.5 mM glucose. These results suggest that glutamine can be a major energy source for cells grown in vitro.
H R Zielke; P T Ozand; J T Tildon; D A Sevdalian; M Cornblath
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  73     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-01-29     Completed Date:  1977-01-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4110-4     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cells, Cultured
Culture Media
Glucose / metabolism*
Glutamates / metabolism
Glutamine / metabolism*
Hypoxanthines / metabolism
Lactates / metabolism
Lesch-Nyhan Syndrome / metabolism
Thymidine / metabolism
Uridine / metabolism
Reg. No./Substance:
0/Culture Media; 0/Glutamates; 0/Hypoxanthines; 0/Lactates; 50-89-5/Thymidine; 50-99-7/Glucose; 56-85-9/Glutamine; 58-96-8/Uridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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