Document Detail

Growth hormone-releasing hormone in testicular interstitial and germ cells: potential paracrine modulation of follicle-stimulating hormone action on Sertoli cell function.
MedLine Citation:
PMID:  7720679     Owner:  NLM     Status:  MEDLINE    
GH-releasing hormone (GHRH) is present in the interstitial and germ cells of the rat testis. In previous studies we found that GHRH is secreted from rat adult Leydig cells, in which it stimulates basal and LH-induced cAMP formation and steroidogenesis. In other studies cAMP production in Sertoli cells was found to be stimulated by GHRH. In the present report, we describe a potential paracrine action of GHRH in the Sertoli cell, with stimulation of cAMP formation in cultured adult and pubertal Sertoli cells. GHRH increased FSH-stimulated cAMP production in adult and pubertal cultures in a time-dependent manner. GHRH stimulation of basal and FSH-induced extracellular cAMP formation was more prominent in pubertal than in adult cultures. Immunocytochemical studies demonstrated the presence of GHRH-like immunoreactivity in rat interstitial cells from day 4 to adult life and in the acrosomal region of early and intermediate spermatids at stages III-VI of the seminiferous epithelium cycle. Immunoreactive GHRH was not observed in late spermatids and mature sperm or in Sertoli cells at any age. These results indicate that GHRH acts synergistically with FSH to promote cAMP production in Sertoli cells in culture. Testicular GHRH of Leydig and germ cell origin may be an important paracrine regulator of Sertoli cell function. Alternatively, GHRH present in germ cells may exert stage-specific intracrine functions.
A Fabbri; D R Ciocca; T Ciampani; J Wang; M L Dufau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  136     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1995 May 
Date Detail:
Created Date:  1995-05-23     Completed Date:  1995-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2303-8     Citation Subset:  AIM; IM    
Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Aging / physiology
Cells, Cultured
Cyclic AMP / metabolism*
Drug Interactions
Follicle Stimulating Hormone / pharmacology*
Growth Hormone-Releasing Hormone / analysis,  pharmacology*,  physiology*
Rats, Sprague-Dawley
Sertoli Cells / cytology,  drug effects,  physiology*
Sexual Maturation*
Spermatids / drug effects,  physiology
Testis / cytology,  growth & development,  physiology*
Reg. No./Substance:
0/Antibodies; 60-92-4/Cyclic AMP; 9002-68-0/Follicle Stimulating Hormone; 9034-39-3/Growth Hormone-Releasing Hormone

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