| Growth hormone-releasing hormone in testicular interstitial and germ cells: potential paracrine modulation of follicle-stimulating hormone action on Sertoli cell function. | |
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MedLine Citation:
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PMID: 7720679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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GH-releasing hormone (GHRH) is present in the interstitial and germ cells of the rat testis. In previous studies we found that GHRH is secreted from rat adult Leydig cells, in which it stimulates basal and LH-induced cAMP formation and steroidogenesis. In other studies cAMP production in Sertoli cells was found to be stimulated by GHRH. In the present report, we describe a potential paracrine action of GHRH in the Sertoli cell, with stimulation of cAMP formation in cultured adult and pubertal Sertoli cells. GHRH increased FSH-stimulated cAMP production in adult and pubertal cultures in a time-dependent manner. GHRH stimulation of basal and FSH-induced extracellular cAMP formation was more prominent in pubertal than in adult cultures. Immunocytochemical studies demonstrated the presence of GHRH-like immunoreactivity in rat interstitial cells from day 4 to adult life and in the acrosomal region of early and intermediate spermatids at stages III-VI of the seminiferous epithelium cycle. Immunoreactive GHRH was not observed in late spermatids and mature sperm or in Sertoli cells at any age. These results indicate that GHRH acts synergistically with FSH to promote cAMP production in Sertoli cells in culture. Testicular GHRH of Leydig and germ cell origin may be an important paracrine regulator of Sertoli cell function. Alternatively, GHRH present in germ cells may exert stage-specific intracrine functions. |
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Authors:
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A Fabbri; D R Ciocca; T Ciampani; J Wang; M L Dufau |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 136 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-05-23 Completed Date: 1995-05-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2303-8 Citation Subset: AIM; IM |
Affiliation:
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Section on Molecular Endocrinology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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physiology Animals Antibodies Cells, Cultured Cyclic AMP / metabolism* Drug Interactions Follicle Stimulating Hormone / pharmacology* Growth Hormone-Releasing Hormone / analysis, pharmacology*, physiology* Immunohistochemistry Male Rats Rats, Sprague-Dawley Sertoli Cells / cytology, drug effects, physiology* Sexual Maturation* Spermatids / drug effects, physiology Testis / cytology, growth & development, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 60-92-4/Cyclic AMP; 9002-68-0/Follicle Stimulating Hormone; 9034-39-3/Growth Hormone-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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