Document Detail


Growth hormone increases ovarian levels of immunoreactive somatomedin C/insulin-like growth factor I in vivo.
MedLine Citation:
PMID:  3943494     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hypothesis that GH may affect gonadal function by increasing local levels of the GH-dependent somatomedin C/insulin-like growth factor I (IGF I) was tested. Ovine GH (200 micrograms) was injected into immature, hypophysectomized, estrogen-treated female rats; animals were sacrificed 8 or 12 h later. Renal and ovarian homogenates were acid extracted and chromatographed over Sephadex G-50. Eight h after GH injection, 3.6 to 6.4-fold increases in immunoreactive IGF I (IR-IGF I) levels were observed in either ovarian or renal extracts subjected to acid chromatography. Twelve h after GH treatment, IR-IGF I levels remained elevated, but were lower than after 8 h. In neither case could IR-IGF I levels be accounted for by serum contamination. IR-IGF I eluted with an apparent mol wt near that of synthetic human IGF I in both kidney and ovary. Thus, GH can directly increase ovarian and renal tissue IR-IGF I levels in vivo. Taken with previous observations showing a direct gonadotropin-enhancing effect of IGF I on rat granulosa cells in vitro, our results support the hypothesis that GH may affect ovarian differentiation by inducing the local production or accumulation of IGF I, providing evidence for a novel intraovarian paracrine control mechanism.
Authors:
J B Davoren; A J Hsueh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrinology     Volume:  118     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1986 Feb 
Date Detail:
Created Date:  1986-03-05     Completed Date:  1986-03-05     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  888-90     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography
Female
Growth Hormone / pharmacology*
Hypophysectomy
Insulin-Like Growth Factor I / blood,  metabolism*
Kidney / drug effects,  metabolism
Kinetics
Ovary / drug effects,  metabolism*
Rats
Rats, Inbred Strains
Somatomedins / metabolism*
Chemical
Reg. No./Substance:
0/Somatomedins; 67763-96-6/Insulin-Like Growth Factor I; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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