Document Detail


Growth hormone increases lung microvascular injury in lipopolysaccharide peritonitis rats: possible involvement of NF-kappaB activation in circulating neutrophils.
MedLine Citation:
PMID:  12370093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the effects of growth hormone (GH) on NF-kappaB activity in neutrophils and neutrophils-mediated organ injury induced by lipopolysaccharide (LPS) in rats. METHODS: Male Wistar rats challenged with or without LPS (5 mg/kg) were treated with varied doses of GH (0.5, 1.0, and 2.0 mg/kg) for 2 or 4 h. NF-kappaB activities in circulating neutrophils were measured with electrophoretic mobility shift assays (EMSA), and I-kappaB levels in circulating neutrophils were detected by Western blot. Lung neutrophils sequestration and lung microvascular permeability were measured at 4 h after LPS challenge. RESULTS: Circulating neutrophils in LPS challenged rats had increased NF-kappaB activity and decreased I-kappaB level as compared with controls. GH dramatically increased NF-kappaB activity and I-kappaB degradation induced by LPS challenge in neutrophils. Also, subsequently, GH treatment increased lung neutrophils sequestration and lung microvascular injury induced by LPS. CONCLUSION: These results suggest that treatment of GH is harmful, instead of beneficial, to LPS-induced organ injury. Increased neutrophils' NF-kappaB activity and lung neutrophils sequestration are critical in vivo mechanisms mediating GH action on LPS-induced organ injury.
Authors:
Zhi-Hai Liu; Ying-Qun Yu; Wei-Qin Li; Jie-Shou Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  23     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-08     Completed Date:  2003-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  887-92     Citation Subset:  IM    
Affiliation:
Research Institute of General Surgery, Nanjing University School of Medicine, Nanjing 210002, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Capillary Permeability / drug effects
Growth Substances / adverse effects*
I-kappa B Proteins / blood
Lipopolysaccharides
Lung Diseases / chemically induced*,  physiopathology
Male
Microcirculation / drug effects*
NF-kappa B / blood,  metabolism*
Neutrophils / physiology*
Peritonitis / blood,  chemically induced,  metabolism,  physiopathology*
Random Allocation
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Growth Substances; 0/I-kappa B Proteins; 0/Lipopolysaccharides; 0/NF-kappa B

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