Document Detail


Growth hormone expression and neuroprotective activity in a quail neural retina cell line.
MedLine Citation:
PMID:  19539627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that growth hormone (GH) is produced within cells of the chick embryo retina where it appears to act as an autocrine/paracrine anti-apoptotic factor in the regulation of programmed cell death during retinal development. These investigations were carried out on cultured chick embryo retinal ganglion cells (RGCs) as well as on the chick embryo retina in ovo, using GH protein knock-down by immunoneutralization. We have now investigated the putative neuroprotective actions of GH using a quail embryo neural retina cell line (QNR/D) treated with GH siRNA to silence the local synthesis of GH. We now show that knock-down of GH by gene silencing in cells of this cultured embryonic neural retina cell line, using NR-cGH-1 siRNA, correlates with the increased appearance in the cultures of cells with apoptotic nuclear morphology. This result is consistent with our previous results using protein knock-down by immunoneutralization. We thus validate, using different technology and a different culture system, our contention that GH, produced locally by cells of the neural retina acts in an autocrine or paracrine manner to regulate cell survival in the retina.
Authors:
Esmond J Sanders; Wan-Ying Lin; Eve Parker; Steve Harvey
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-17
Journal Detail:
Title:  General and comparative endocrinology     Volume:  165     ISSN:  1095-6840     ISO Abbreviation:  Gen. Comp. Endocrinol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-11-20     Completed Date:  2010-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370735     Medline TA:  Gen Comp Endocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  111-9     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Alberta, Edmonton, Alberta, Canada. esmond.sanders@ualberta.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics,  physiology
Blotting, Western
Cell Line
Chick Embryo
Chickens
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation*
Growth Hormone / genetics,  metabolism*
Pituitary Gland / metabolism
RNA, Small Interfering
Retina / cytology*,  embryology,  metabolism*
Retinal Ganglion Cells / cytology,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/RNA, Small Interfering; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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