Document Detail

Growth hormone-dependent tyrosine phosphorylation of a GH receptor-associated high molecular WEIGHT protein immunologically related to JAK2.
MedLine Citation:
PMID:  9918803     Owner:  NLM     Status:  MEDLINE    
A critical step in growth hormone (GH) signalling is the GH-induced activation of the GH receptor (GHR)-associated tyrosine kinase, JAK2. JAK2 is a 120 kD member of the Janus family of tyrosine kinases, whose other mammalian members include JAK1, JAK3, and TYK2. Using 3T3-F442A murine preadipocytes, we now report detection of a Mr approximately 170 kD protein, referred to as HMW ("high molecular weight") JAK2, that is specifically reactive in immunoprecipitation and immunoblotting experiments with three independently-derived anti-JAK2 antibodies--two directed at carboxyl-terminal regions of the molecule and one directed at the amino-terminus. Like JAK2, HMW JAK2 is tyrosine phosphorylated in response to GH treatment of cells and is coimmunoprecipitated with anti-GHR serum. Thus, HMW JAK2 is a protein not heretofore described that is immunologically related to JAK2 and is physically and functionally associated with the GHR.
J Jiang; L Liang; S O Kim; Y Zhang; R Mandler; S J Frank
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  253     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-09     Completed Date:  1999-02-09     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  774-9     Citation Subset:  IM    
Department of Medicine, University of Alabama at Birmingham, Veterans Affairs Medical Center, 35294, USA.
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MeSH Terms
3T3 Cells
Adipocytes / cytology,  metabolism
Cross Reactions
Janus Kinase 2
Molecular Weight
Peptide Fragments / immunology
Phosphoproteins / immunology,  metabolism
Precipitin Tests
Protein Binding
Protein-Tyrosine Kinases / immunology,  metabolism*
Proto-Oncogene Proteins*
Receptors, Somatotropin / metabolism*
Signal Transduction
Stem Cells / cytology,  metabolism
Tyrosine / metabolism
Grant Support
Reg. No./Substance:
0/Peptide Fragments; 0/Phosphoproteins; 0/Proto-Oncogene Proteins; 0/Receptors, Somatotropin; 55520-40-6/Tyrosine; EC Kinase 2; EC Kinases; EC protein, mouse

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