Document Detail


Growth and fatty acid composition of Nannochloropsis sp. grown mixotrophically in fed-batch culture.
MedLine Citation:
PMID:  15604757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell growth and eicosapentaenoic acid (EPA) yields of Nannochloropsis sp. were enhanced in the fed-batch cultures. With feeding glucose solution, the biomass reached 1.1 g dry wt l(-1) after 10 days' culture, which was 40% higher than that obtained in the batch culture (0.8 g dry wt l(-1)). With supplement of nitrate solution, the biomass reached 1 g dry wt l(-1), and reached the stationary phase 2 days earlier than the others. The maximum of biomass (1.2 g dry wt l(-1)) was obtained with the supplement of the mixture of glucose and nitrate solution. The EPA yields of Nannochloropsis sp. after 10 days' growth in the fed-batch cultures were 52 mg l(-1), 43 mg l(-1) and 56 mg l(-1) with, respectively, addition of nitrate, glucose and both together. In batch culture only 35 mg EPA l(-1) was obtained.
Authors:
Fang Xu; Zhao-Ling Cai; Wei Cong; Fan Ouyang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-12-17     Completed Date:  2005-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1319-22     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P.O. Box 353, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Algae / growth & development*,  metabolism*
Animals
Biomass
Bioreactors
Eicosapentaenoic Acid / biosynthesis*,  isolation & purification
Glucose / metabolism
Nitrates / metabolism
Chemical
Reg. No./Substance:
0/Nitrates; 1553-41-9/Eicosapentaenoic Acid; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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