| Growth factor production from fibrin-encapsulated human keratinocytes. | |
| | |
MedLine Citation:
|
PMID: 20349112 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-beta1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-beta1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-beta1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-beta1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors. |
| | |
Authors:
|
Cristian A Acevedo; Rodrigo A Somoza; Caroline Weinstein-Oppenheimer; Donald I Brown; Manuel E Young |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-27 |
Journal Detail:
|
Title: Biotechnology letters Volume: 32 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-17 Completed Date: 2010-09-15 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 1011-7 Citation Subset: IM |
Affiliation:
|
Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile. cristian.acevedo@usm.cl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Culture Techniques Cells, Cultured Fibrin / metabolism* Humans Intercellular Signaling Peptides and Proteins / secretion* Keratinocytes / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Intercellular Signaling Peptides and Proteins; 9001-31-4/Fibrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Antitumor activity of methylan polysaccharide derivatives.
Next Document: Glutathione-mediated response to acid stress in the probiotic bacterium, Lactobacillus salivarius.