Document Detail


Growth and electrophysiological properties of rat embryonic cardiomyocytes on hydroxyl- and carboxyl-modified surfaces.
MedLine Citation:
PMID:  18854125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biodegradable scaffolds such as poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA) or poly(glycolic acid) (PGA) are commonly used materials in tissue engineering. The chemical composition of these scaffolds changes during degradation which provides a differential environment for the seeded cells. In this study we have developed a simple and relatively high-throughput method in order to test the physiological effects of this varying chemical environment on rat embryonic cardiac myocytes. In order to model the different degradation stages of the scaffold, glass coverslips were functionalized with 11-mercaptoundecanoic acid (MUA) and 11-mercapto-1-undecanol (MUL) as carboxyl- and hydroxyl-groups presenting surfaces, and with trimethoxysilylpropyldiethylenetriamine (DETA) and (3-aminopropyl)triethoxysilane (APTES) as controls. Embryonic cardiac myocytes formed beating islands on all tested surfaces, but the number of attached cells and beating patches was significantly lower on MUL compared to any of the other functionalized surfaces. Moreover, whole-cell patch-clamp experiments showed that the average length of action potentials generated by the beating-cardiac myocytes were significantly longer on MUL compared to the other surfaces. Our results, using our simple test system, are in basic agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing could be a relatively high throughput method for biocompatibility studies and for the optimization of the material/tissue interface in tissue engineering.
Authors:
Anupama Natarajan; Changju Chun; James J Hickman; Peter Molnar
Related Documents :
19810675 - Glucose-responsive micelles from self-assembly of poly(ethylene glycol)-b-poly(acrylic ...
7738265 - Effects of several tea components on acid resistance of human tooth enamel.
3482345 - Biochemical and scanning electron microscope study of lipids chloroform-methanol extrac...
22974045 - Intake of omega-3 and omega-6 fatty acids and risk of basal and squamous cell carcinoma...
23330845 - Universal chemiluminescence flow-through device based on directed self-assembly of soli...
23419415 - Free fatty acids in the cuticular and internal lipids of calliphora vomitoria and their...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  19     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2008  
Date Detail:
Created Date:  2008-10-15     Completed Date:  2009-03-11     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1319-31     Citation Subset:  IM    
Affiliation:
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Dioxide / chemistry*,  pharmacology*
Cell Proliferation / drug effects
Culture Media
Electrophysiological Processes / drug effects*
Hydroxides / chemistry*,  pharmacology*
Myocytes, Cardiac / cytology*,  drug effects,  physiology*
Rats
Solubility
Surface Properties
Grant Support
ID/Acronym/Agency:
K01 EB003465-03/EB/NIBIB NIH HHS; R21 EB 002307-02/EB/NIBIB NIH HHS; R21 EB002307-02/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Hydroxides; 124-38-9/Carbon Dioxide; 14485-07-5/carboxyl radical; 9159UV381P/hydroxide ion
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabri...
Next Document:  Mechanistic analysis of macrophage response to IRAK-1 gene knockdown by a smart polymer-antisense ol...