Document Detail

Growth and electrophysiological properties of rat embryonic cardiomyocytes on hydroxyl- and carboxyl-modified surfaces.
MedLine Citation:
PMID:  18854125     Owner:  NLM     Status:  MEDLINE    
Biodegradable scaffolds such as poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA) or poly(glycolic acid) (PGA) are commonly used materials in tissue engineering. The chemical composition of these scaffolds changes during degradation which provides a differential environment for the seeded cells. In this study we have developed a simple and relatively high-throughput method in order to test the physiological effects of this varying chemical environment on rat embryonic cardiac myocytes. In order to model the different degradation stages of the scaffold, glass coverslips were functionalized with 11-mercaptoundecanoic acid (MUA) and 11-mercapto-1-undecanol (MUL) as carboxyl- and hydroxyl-groups presenting surfaces, and with trimethoxysilylpropyldiethylenetriamine (DETA) and (3-aminopropyl)triethoxysilane (APTES) as controls. Embryonic cardiac myocytes formed beating islands on all tested surfaces, but the number of attached cells and beating patches was significantly lower on MUL compared to any of the other functionalized surfaces. Moreover, whole-cell patch-clamp experiments showed that the average length of action potentials generated by the beating-cardiac myocytes were significantly longer on MUL compared to the other surfaces. Our results, using our simple test system, are in basic agreement with earlier observations that utilized a complex 3D biodegradable scaffold. Thus, surface functionalization with self-assembled monolayers combined with histological/physiological testing could be a relatively high throughput method for biocompatibility studies and for the optimization of the material/tissue interface in tissue engineering.
Anupama Natarajan; Changju Chun; James J Hickman; Peter Molnar
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  19     ISSN:  0920-5063     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2008  
Date Detail:
Created Date:  2008-10-15     Completed Date:  2009-03-11     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1319-31     Citation Subset:  IM    
NanoScience Technology Center, University of Central Florida, 12424 Research Parkway, Orlando, FL 32826, USA.
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MeSH Terms
Carbon Dioxide / chemistry*,  pharmacology*
Cell Proliferation / drug effects
Culture Media
Electrophysiological Processes / drug effects*
Hydroxides / chemistry*,  pharmacology*
Myocytes, Cardiac / cytology*,  drug effects,  physiology*
Surface Properties
Grant Support
K01 EB003465-03/EB/NIBIB NIH HHS; R21 EB 002307-02/EB/NIBIB NIH HHS; R21 EB002307-02/EB/NIBIB NIH HHS
Reg. No./Substance:
0/Culture Media; 0/Hydroxides; 124-38-9/Carbon Dioxide; 14485-07-5/carboxyl radical; 9159UV381P/hydroxide ion

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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