Document Detail

Growth and differentiation of PC6 cells: the effects of pulsed electromagnetic fields (PEMF).
MedLine Citation:
PMID:  11298388     Owner:  NLM     Status:  MEDLINE    
Previous studies in our laboratory showed that neurite outgrowth in vitro and nerve regeneration in vivo were stimulated by 2 Hz, 0.3 mT (3 G) pulsed electromagnetic fields (PEMF). To learn more about the effects of PEMF on nerve cells, we exposed PC6 cells, a standard neuronal-like cell model, to the same pulsed electromagnetic fields for 2 h/day for 2 days and asked whether two different cell processes, proliferation and differentiation, were affected. The cells were also treated with a differentiating agent, nerve growth factor (NGF), to further define any interactive effects. We found that proliferation was unaffected by either PEMF or NGF alone or in combination. Differentiation, expressed as neurite outgrowth, was strongly upregulated with NGF, but this NGF response was significantly depressed in cells treated with PEMF.
J P Shah; P Midkiff; P C Brandt; B F Sisken
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioelectromagnetics     Volume:  22     ISSN:  0197-8462     ISO Abbreviation:  Bioelectromagnetics     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-12     Completed Date:  2001-07-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008281     Medline TA:  Bioelectromagnetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  267-71     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Center for Biomedical Engineering and Department of Anatomy and Neurobiology, University of Kentucky, Lexington 40506-0070, USA.
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MeSH Terms
Cell Differentiation / drug effects,  radiation effects*
Cell Division / drug effects,  radiation effects*
Electromagnetic Fields*
Models, Neurological
Nerve Growth Factor / pharmacology
Neurons / cytology*,  drug effects,  radiation effects
PC12 Cells
Time Factors
Reg. No./Substance:
9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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