Document Detail

Growth and development of haemopoietic cells: a deterministic process?
MedLine Citation:
PMID:  2696678     Owner:  NLM     Status:  MEDLINE    
The in vitro methods used to study haemopoiesis fall into two distinct categories. Short-term colony forming assays have identified a number of potent soluble factors capable of maintaining survival, proliferation and differentiation of haemopoietic cells but not their self-renewal. In contrast, in long-term bone marrow culture, extensive self-renewal occurs in the absence of exogenous factors and direct physical contact between haemopoietic cells and cells of the adherent stromal layer seems to be important. Obviously, LTBMC more closely resembles the situation in haemopoietic tissues but the potency of growth factors imply that they too play a role. Our data suggest that this may be at an earlier stage of haemopoietic development than previously appreciated. Primitive multipotent cells have the potential to respond to CSFs which were previously thought to stimulate only committed progenitor cells. This response is only seen, however, when the cells are exposed to a combination of factors which include either IL-1 or G-CSF. Thus, a combination of factors is able to recruit cells which are not already committed and determine the lineage along which they will differentiate. While it remains to be conclusively demonstrated that growth factors regulate normal "steady state" haemopoiesis in vivo it is clear that contact with stromal cells is important. The mechanisms by which the adherent layer influences haemopoietic development, however, are less obvious. We have shown that a component of stroma, heparan sulphate, is able to bind growth factors and present them to haemopoietic cells in a way that stimulates haemopoiesis.(ABSTRACT TRUNCATED AT 250 WORDS)
C P Daniel; I L Ponting; T M Dexter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Haematology and blood transfusion     Volume:  32     ISSN:  0171-7111     ISO Abbreviation:  Haematol Blood Transfus     Publication Date:  1989  
Date Detail:
Created Date:  1990-03-30     Completed Date:  1990-03-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101169459     Medline TA:  Haematol Blood Transfus     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  172-7     Citation Subset:  IM    
Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK.
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MeSH Terms
Bone Marrow Cells
Cell Differentiation / physiology
Cell Division / physiology
Cells, Cultured
Colony-Stimulating Factors / physiology
Hematopoietic Stem Cells / cytology*
Reg. No./Substance:
0/Colony-Stimulating Factors

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