| Growth, cultural characteristics, and antibacterial sensitivity of Leptospira interrogans serovar hardjo. | |
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MedLine Citation:
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PMID: 6884034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nutrient requirements of Leptospira interrogans serovar hardjo were investigated using a 1% bovine serum albumin (BSA) medium, supplemented separately with polysorbates (Tweens) 80, 60, 40, 20, NH4Cl, and vitamins thiamine and cyanocobalamin (vitamin B12). L. hardjo was tested in vitro against 30 antibacterial compounds incorporated into semisolid medium (0.2% agar) at 3 compound concentrations. Growth was superior in polysorbate 80 (oleic acid rich) and polysorbate 40 (palmitic rich) media. A linear growth response to vitamin B12 could be shown. Hamster isolates all required thiamine and vitamin B12 for growth. The antibacterial compounds could be classed as: 100% bacteriocidal, bacteriocidal at the 2 highest concentrations, bacteriocidal at only 1 concentration, and completely noninhibitory. The tetracyclines were strongly bacteriocidal. The comparative growth rates of hamster isolates were much reduced when compared to the type strain hardjoprajitno. These studies were performed acknowledging that the polysorbates were mixtures of fatty acids. Nephelometry was used as an accepted method to monitor the synthesis of leptospiral cell mass. |
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Authors:
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H C Ellinghausen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Cornell veterinarian Volume: 73 ISSN: 0010-8901 ISO Abbreviation: Cornell Vet Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1983-10-21 Completed Date: 1983-10-21 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0074245 Medline TA: Cornell Vet Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 225-39 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Bacterial Agents / pharmacology Cattle Cricetinae Culture Media Drug Resistance, Microbial Leptospira interrogans / drug effects, growth & development* Polysorbates / pharmacology Rabbits Serum Albumin, Bovine Thiamine / pharmacology Vitamin B 12 / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Culture Media; 0/Polysorbates; 0/Serum Albumin, Bovine; 59-43-8/Thiamine; 68-19-9/Vitamin B 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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