| Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro. | |
| | |
MedLine Citation:
|
PMID: 7000727 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The in vitro growth characteristics and morphology of human retinal pigment epithelium (RPE) cells from adult donor eyes (15 to 100 years of age) have been studied. Although RPE cells are viable in culture for several months, only a fraction of the cells actually divide. Rapid cell proliferation and confluency of a culture occur from 10 to 30 days after seeding. The time for confluency is both age- and media-dependent; the number of cells that are potential dividers and contribute to confluency decreases with increasing donor age. Since melanolipofuscin granules do not form in vitro and are diluted by cell division, stationary (nondividing) cells can be distinguished from the dividing cells by the presence of dense clusters of melanolipofuscin granules in the stationary cells. Confluent cultures contain a monolayer of relatively clear polygonal cells with densely pigmented stationary cells scattered throughout. Stationary cells can often represent as much as 95% of the original RPE cell population. Ultrastructurally all cells appear epithelioid, with apical-basal polarity, junctional complexes, and cytoplasmic organization characteristic of RPE cells in vivo. Stationary cells are extremely large, with melanolipofuscin granules clustered around the nucleus. Melanolipofuscin is a unique marker for nondividing cells and may facilitate studies of the age-dependent loss of replicability of human RPE. |
| | |
Authors:
|
M T Flood; P Gouras; H Kjeldbye |
Related Documents
:
|
12883667 - Adenoviral p16/cdkn2 gene transfer to malignant glioma: role of p16 in growth, invasion... 22154697 - P21, an important mediator of quiescence during pituitary tumor formation, is dispensab... 17183247 - Accelerated expression of senescence associated cell cycle inhibitor p16ink4a in kidney... 9719027 - Telomeric dna: marker for human prostate cancer development? 3403547 - A possible role for plasmalogens in protecting animal cells against photosensitized kil... 1671407 - Ngf and excitatory neurotransmitters regulate survival and morphogenesis of cultured ce... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 19 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1980 Nov |
Date Detail:
|
Created Date: 1981-01-26 Completed Date: 1981-01-26 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1309-20 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Age Factors Aged Cell Division Humans Microscopy, Electron Microscopy, Phase-Contrast Middle Aged Pigment Epithelium of Eye / cytology, growth & development*, ultrastructure Pigments, Biological |
| Grant Support | |
ID/Acronym/Agency:
|
EY 02591/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Pigments, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lethal effect after transmutation of 33P incorporated into bacteriophage S 13 and mechanisms of DNA ...
Next Document: Prolonged survival of allogeneic mouse skin grafts following preservation.