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Growth characteristics of poliovirus in HeLa cells: nucleic acid metabolism.
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MedLine Citation:
PMID:  13475620     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The RNA and DNA contents of the nucleus and cytoplasm of the HeLa cell were determined. The rates of incorporation of P(32) into the various nucleic acid fractions were established for the ordinary HeLa cell maintained under a set of standard conditions. The changes in the rates of incorporation of P(32) and in the amounts of RNA and DNA which occurred subsequent to infection with poliovirus were followed throughout the infectious cycle. These changes were correlated with the intracellular appearance of the newly formed virus. A net synthesis of RNA occurred in the cytoplasmic component of the cell. The increase was detectable 2 hours before the first appearance of demonstrable virus and reached a maximum (2.5 times normal) at 6 hours. Viral increase was not maximal before the 7th hour after infection.
Authors:
H F MAASSAB; P C LOH; W W ACKERMANN
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  106     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1957 Nov 
Date Detail:
Created Date:  1958-12-01     Completed Date:  2000-07-01     Revised Date:  2012-04-16    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  641-8     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Nucleic Acids / metabolism*
Poliovirus / ethnology*
Chemical
Reg. No./Substance:
0/Nucleic Acids
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1957, by The Rockefeller Institute for Medical Research New York
Received Day: 22 Month: 6 Year: 1957
Print publication date: Day: 31 Month: 10 Year: 1957
Volume: 106 Issue: 5
First Page: 641 Last Page: 648
ID: 2136822
PubMed Id: 13475620

GROWTH CHARACTERISTICS OF POLIOVIRUS IN HELA CELLS: NUCLEIC ACID METABOLISM
H. F. Maassab
Philip C. Loh
W. Wilbur Ackermann
From the Department of Epidemiology and Virus Laboratory, School of Public Health, University of Michigan, Ann Arbor


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