Document Detail


Growth characteristics of anaerobically treated Ehrlich ascites tumor cells after reaeration as studied by combination of flow cytometry and centrifugal elutriation.
MedLine Citation:
PMID:  7136187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Centrifugal elutriation was applied to separate into the cell compartments asynchronous Ehrlich ascites cells grown under different culture conditions. The cytokinetic properties of the recultivated fractions were studied by flow cytometry. The present experiments prove that G1-cohorts grown 12 h under exclusion of oxygen accumulate in the late G1-compartment. The cytokinetic properties of these cells are not changed. In contrast the cytokinetic properties of anaerobically treated S-phase cells are changed; most of S-cells leave the cell cycle after they have attained the DNA content of G2-cells and continue to synthesize DNA without preceeding division. Cells with a DNA content up to the fourfold of normal values are found.
Authors:
R Merz; F Schneider
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. Section C: Biosciences     Volume:  37     ISSN:  0341-0382     ISO Abbreviation:  Z. Naturforsch., C, Biosci.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2009-06-08    
Medline Journal Info:
Nlm Unique ID:  7801143     Medline TA:  Z Naturforsch C     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Animals
Carcinoma, Ehrlich Tumor / physiopathology*
Cell Cycle
Cell Division
Centrifugation
DNA, Neoplasm / analysis
Flow Cytometry
Interphase
Kinetics
Mice
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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