Document Detail

Growth characteristics of anaerobically treated Ehrlich ascites tumor cells after reaeration as studied by combination of flow cytometry and centrifugal elutriation.
MedLine Citation:
PMID:  7136187     Owner:  NLM     Status:  MEDLINE    
Centrifugal elutriation was applied to separate into the cell compartments asynchronous Ehrlich ascites cells grown under different culture conditions. The cytokinetic properties of the recultivated fractions were studied by flow cytometry. The present experiments prove that G1-cohorts grown 12 h under exclusion of oxygen accumulate in the late G1-compartment. The cytokinetic properties of these cells are not changed. In contrast the cytokinetic properties of anaerobically treated S-phase cells are changed; most of S-cells leave the cell cycle after they have attained the DNA content of G2-cells and continue to synthesize DNA without preceeding division. Cells with a DNA content up to the fourfold of normal values are found.
R Merz; F Schneider
Related Documents :
6151287 - Cytochemical localization of adenyl cyclase in isoproterenol stimulated hepatoma ascite...
8920757 - Bioassay for determining 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (tes) in human...
7127297 - Ascites tumor invasion of mouse peritoneum studied by high-voltage electron microscope ...
7553597 - Gene therapy of hepatoma: bystander effects and non-apoptotic cell death induced by thy...
23727117 - Comparison of the sensitivities of three commercial assays for detection of the high ri...
20882667 - V-region gene analysis of locally defined synovial b and plasma cells reveals selected ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zeitschrift für Naturforschung. Section C: Biosciences     Volume:  37     ISSN:  0341-0382     ISO Abbreviation:  Z. Naturforsch., C, Biosci.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1982-12-18     Completed Date:  1982-12-18     Revised Date:  2009-06-08    
Medline Journal Info:
Nlm Unique ID:  7801143     Medline TA:  Z Naturforsch C     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  833-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carcinoma, Ehrlich Tumor / physiopathology*
Cell Cycle
Cell Division
DNA, Neoplasm / analysis
Flow Cytometry
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The interaction of calf thymus DNA with mercuric acetate and 3,6-bis-(acetatomercurimethyl)-dioxane....
Next Document:  The sporulation of Eimeria tenella as revealed by a novel preparative method.