Document Detail

Growth of canine T-lymphocyte colonies in vitro.
MedLine Citation:
PMID:  307494     Owner:  NLM     Status:  MEDLINE    
Canine lymphocytes from peripheral blood, lymph nodes, thymus and bone marrow were stimulated with phytohemagglutinin-P (PHA) or concanavalin-A (CON-A) to form colonies in methylcellulose. Lymphocytes exposed to mitogens in liquid phase formed clumps the size of colonies. Lymphocyte clumping was eliminated by plating cells directly into methylcellulose, but high concentrations of mitogens (CON-A or PHA is greater than 10 mg/10(6) lymphocytes) were required in order to get subsequent colony formation. Thus, in contrast to published reports, exposure of lymphocytes to mitogen prior to plating was not required for cloning of canine peripheral blood lymphocytes. Colonies from thymus, lymph node, or peripheral blood consisted predominantly of T lymphocytes, whereas cultures from bone marrow also produced colonies with macrophage morphology and surface-adherent colonies with mesenchymal morphology.
F D Wilson; M Shifrine; M E Gershwin; W Spangler; J Dyck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental hematology     Volume:  6     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-30     Completed Date:  1978-09-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  539-48     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cells, Cultured
Clone Cells
Lymphocyte Activation
T-Lymphocytes / cytology*,  ultrastructure
Reg. No./Substance:

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