| Growth suppression of human laryngeal squamous cell carcinoma by adenoviral-mediated interleukin-12. | |
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MedLine Citation:
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PMID: 20819436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study explored the inhibitory role of the adenoviral-mediated-interleukin (IL)-12 (Ad.mIL-12) gene in the growth of laryngeal squamous cell carcinoma (LSCC). Human epithelial type 2 (Hep-2) cells were transfected with Ad.mIL-12, and IL-12 gene expression of the cells was evaluated. The proliferation and apoptosis of Hep-2 cells in vitro were detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Experimental tumours in mice were injected intratumourally with the same recombinant adenoviruses and inhibition of tumour growth observed. Apoptosis in Hep-2 xenotransplants was detected using TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labelling) assay and transmission electron microscopy. The expression of IL-12 in Ad.mIL-12 transfected Hep-2 cells was significantly increased. In vitro, Ad.mIL-12 decreased the viability of and increased apoptosis in Hep-2 cells. Increased apoptosis was also seen in vivo. The mean weight and volume of tumours in Ad.mIL-12 treated mice were significantly lower than in the control group. It is concluded that Ad.mIL-12 can suppress LSCC growth and induce apoptosis. |
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Authors:
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L Tian; X Chen; Y Sun; M Liu; D Zhu; J Ren |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of international medical research Volume: 38 ISSN: 0300-0605 ISO Abbreviation: J. Int. Med. Res. Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-09-07 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0346411 Medline TA: J Int Med Res Country: England |
Other Details:
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Languages: eng Pagination: 994-1004 Citation Subset: IM |
Affiliation:
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Department of Otorhinolaryngology/Head and Neck Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Animals Apoptosis Carcinoma, Squamous Cell / metabolism, pathology*, therapy Cell Survival Gene Therapy / methods* Genetic Vectors* Hep G2 Cells / ultrastructure, virology Humans Interleukin-12 / genetics, metabolism Laryngeal Neoplasms / metabolism, pathology*, therapy Mice Mice, Inbred BALB C Microscopy, Electron, Transmission Tetrazolium Salts / metabolism Thiazoles / metabolism Transfection Xenograft Model Antitumor Assays |
| Chemical | |
Reg. No./Substance:
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0/Tetrazolium Salts; 0/Thiazoles; 187348-17-0/Interleukin-12; 298-93-1/thiazolyl blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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