Document Detail

Growth of Salmonella during sprouting of alfalfa seeds associated with salmonellosis outbreaks.
MedLine Citation:
PMID:  11357873     Owner:  NLM     Status:  MEDLINE    
Growth of Salmonella was assessed during sprouting of naturally contaminated alfalfa seeds associated with two outbreaks of salmonellosis. Salmonella was determined daily in sprouts and sprout rinse water samples by a three-tube most probable number (MPN) procedure and a commercial enzyme immunoassay (EIA). Growth of Salmonella in the sprouts was reflected in the rinse water, and the MPNs of the two samples were generally in agreement within approximately 1 log. The results from EIA testing of sprouts and water samples were also in agreement. The pathogen was present in the seed at less than 1 MPN/g, and it increased in number to maximum population levels of 102 to 10(3) MPN/g in one seed lot and 10(2) to 10(4) MPN/ g in the other seed lot. Maximum populations of the pathogen were apparent by day 2 of sprouting. These results show the ability of the pathogen to grow to detectable levels during the sprouting process, and they provide support for the recommendation to test the sprout water for the presence of pathogens 48 h after starting seed sprouting. The effectiveness of a 10-min, 20,0000-microg/ml (ppm) calcium hypochlorite treatment of the outbreak-associated seeds was studied. For both seed lots, the hypochlorite treatment caused a reduction, but not elimination, of Salmonella contamination in the finished sprouts. These results confirm the need to test each production batch for the presence of pathogens, even after 20,000 microg/ml (ppm) hypochlorite treatment of seeds, so that contaminated product is not distributed.
D S Stewart; K F Reineke; J M Ulaszek; M L Tortorello
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  64     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-11     Completed Date:  2001-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  618-22     Citation Subset:  IM    
US Food and Drug Administration, National Center for Food Safety and Technology, Summit-Argo, Illinois 60501, USA.
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MeSH Terms
Calcium Compounds / pharmacology*
Colony Count, Microbial
Disease Outbreaks
Immunoenzyme Techniques
Medicago sativa / microbiology*
Salmonella / drug effects,  growth & development*,  pathogenicity
Salmonella Food Poisoning / prevention & control
Seeds / microbiology*
Time Factors
Water Microbiology
Grant Support
FD-0004331/FD/FDA HHS
Reg. No./Substance:
0/Calcium Compounds; 7778-54-3/calcium hypochlorite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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