| Growth Properties and Cholesterol Removal Ability of Electroporated Lactobacillus acidophilus BT 1088. | |
| | |
MedLine Citation:
|
PMID: 22580318 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
This study aimed to evaluate the effects of electroporation on the cell growth, cholesterol removal, and adherence abilities of L. acidophilus BT 1088 and their subsequent passages. The growth of electroporated parent cells increased (P<0.05) by 4.49-21.25% compared with that of the control. This may be attributed to the alteration of cellular membrane. However, growth of first, second, and third passages of treated cells was comparable with that of the control, which may be attributed to the resealing of transient pores on the cellular membrane. Electroporation also increased (P<0.05) assimilation of cholesterol by treated parent cells (>185.40%) and first passage (>21.72%) compared with that of the control. Meanwhile, incorporation of cholesterol into the cellular membrane was also increased (P<0.05) in the treated parent cells (>108.33%) and first passage (>26.67%), accompanied by increased ratio of cholesterol:phospholipids (C:P) in these passages. Such increased ratio was also supported by increased enrichment of cholesterol in the hydrophilic heads, hydrophobic tails, and the interface regions of the membrane phospholipids of both parent and first passage cells compared with that of the control. However, such traits were not inherited by the subsequent second and third passages. Parent cells also showed decreased intestinal adherence ability (P<0.05; decreased by 1.45%) compared with that of the control, without inheritance by subsequent passages of treated cells. Our data suggest that electoporation could be a potential physical treatment to enhance the cholesterol removal ability of lactobacilli that was inherited by the first passage of treated cells without affecting their intestinal adherence ability. |
| | |
Authors:
|
Huey Shi Lye; Boon Yin Khoo; Abdul Alias Karim; Gulam Rusul; Min Tze Liong |
Related Documents
:
|
8667208 - Catecholamine transporters and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxici... 38828 - The low ionic strength reaction of human blood: relationship between the binding of ser... 19577438 - A novel compound n(1),n(5)-(z)-n(10)-(e)-tri-p-coumaroylspermidine isolated from cartha... 5773018 - Arsenic-lipid complex formatinon during the active transport of arsenate in yeast. 12789278 - Microphthalamia-associated transcription factor: a critical regulator of pigment cell d... 2457978 - In vitro translation of rat intestinal rna prepared from isolated villus and crypt cell... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of microbiology and biotechnology Volume: 22 ISSN: 1738-8872 ISO Abbreviation: J. Microbiol. Biotechnol. Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-05-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9431852 Medline TA: J Microbiol Biotechnol Country: Korea (South) |
Other Details:
|
Languages: eng Pagination: 981-9 Citation Subset: IM |
Affiliation:
|
School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of Monacolin K-Enriched Ganghwayakssuk (Artemisia princeps Pamp.) by Fermentation with M...
Next Document: Improved Production of Long-Chain Fatty Acid in Escherichia coli by an Engineering Elongation Cycle ...