| Growth of Porphyromonas gingivalis, Treponema denticola, T. pectinovorum, T. socranskii, and T. vincentii in a chemically defined medium. | |
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MedLine Citation:
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PMID: 1400984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A chemically defined medium, OMIZ (Oral Microbiology and Immunology, Zürich)-W1 was developed. Medium OMIZ-W1 supports the long-term proliferation of a wide range of oral anaerobes, including representative strains of four Treponema species and Porphyromonas gingivalis. High concentrations of ascorbic acid and ammonium ions proved to be important for the growth of these organisms. T. denticola CD-1 grew in the absence of polyamines and long-chain fatty acids, T. pectinovorum and T. socranskii required polyamines, whereas T. vincentii depended on both polyamines and lecithin for growth. Specific requirements for purines and/or pyrimidines were detected, and these requirements could be used to distinguish Haemophilus-Actinobacillus group organisms. Some strains of P. gingivalis grew without vitamin K, while others were not satisfied by menadione but required its precursor 1,4-dihydroxy-2-naphthoic acid. Protoporphyrin IX or hemin equally satisfied the porphyrin requirements of P. gingivalis and Bacteroides forsythus, whereas ferrous sulfate was more efficiently used as a source of iron than was hemin. The cellular cohesiveness of P. gingivalis increased with high concentrations of hemin in the growth medium. Prevotella intermedia, B. forsythus, and several strains of P. gingivalis were more fastidious and required a protein or serum supplement to grow in medium OMIZ-W1. |
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Authors:
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C Wyss |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 30 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1992 Sep |
Date Detail:
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Created Date: 1992-10-30 Completed Date: 1992-10-30 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2225-9 Citation Subset: IM |
Affiliation:
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Department of Oral Microbiology and General Immunology, Dental Institute of the University of Zürich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascorbic Acid
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metabolism Bacteria, Anaerobic / growth & development* Bacteriological Techniques* Cell Adhesion / physiology Culture Media Fatty Acids, Volatile / metabolism Hemin / metabolism Mouth / microbiology* Polyamines / metabolism Quaternary Ammonium Compounds / metabolism Species Specificity Treponema / growth & development* Vitamin K / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids, Volatile; 0/Polyamines; 0/Quaternary Ammonium Compounds; 12001-79-5/Vitamin K; 16009-13-5/Hemin; 50-81-7/Ascorbic Acid |
| Comments/Corrections | |
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