Document Detail


Growth of Ovarian Primary Follicles Retrieved from Neonates of Different Ages and Derivation of Mature Oocytes Following In vitro-Culture.
MedLine Citation:
PMID:  25049606     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follicles of 75 to 99 μm in diameter were collected daily from 7- to 14-day-old neonatal mice, and subsequently cultured in α-MEM medium. Number of primary follicles isolated, growth of the follicle during in vitro-culture and maturation of intrafollicular oocytes were monitored. Overall, mean number of preantral follicles per animal was improved from 10.7 to 88.7 as the age of follicle donors was increased from 7 to 14-day-old. Number of primary follicles was increased gradually up to 11-day-old (35.7 follicle per an animal), then reduced to 29 in 14-day-old (p = 0.0013). More follicles retrieved from 10-day-old or 11-day-old females maintained their morphological normality at the end of primary culture than the follicles retrieved from 9-day-old. Of those cultured, primary follicles retrieved from 11-day-old mice yielded largest larger number of early secondary follicles than the follicles retrieved from in the other ages (39 vs. 13 to 29%). More than 3.3-times increase (0.86 to 2.86; p<0.05) in an average number of mature oocytes per animal was observed in the group of 11-day-old, compared with 9-day-old. However, no difference was found in the percentage of primary follicles developing into the pseudoantral stage (21 to 30%; p = 0.5222) and in the percentage of oocytes mucified (32 to 39%; p = 0.5792). In conclusion, a positive correlation between retrieval time and follicle growth was detected, which influences the efficiency to derive mature oocytes by follicle culture.
Authors:
J H Choi; C R Yoo; J Y Ahn; J H Park; J M Lim
Related Documents :
24960026 - The relationship between early growth and survival of hatchling saltwater crocodiles (c...
23439296 - Use of retinyl acetate, retinoic acid and insulin-like growth factor-i (igf-i) to enhan...
22862896 - Diagnostic yield of tuberculosis using sputum induction in hiv-positive patients before...
25131086 - Impact of hypothermic preservation on tissue yield and viability in pig pancreata.
14675176 - Collagen of chronically inflamed skin is over-modified and upregulates secretion of mat...
12480096 - The microbiology of butyrate formation in the human colon.
Publication Detail:
Type:  Journal Article     Date:  2012-05-01
Journal Detail:
Title:  Asian-Australasian journal of animal sciences     Volume:  25     ISSN:  1011-2367     ISO Abbreviation:  Asian-australas. J. Anim. Sci.     Publication Date:  2012 May 
Date Detail:
Created Date:  2014-07-22     Completed Date:  2014-07-22     Revised Date:  2014-07-28    
Medline Journal Info:
Nlm Unique ID:  9884245     Medline TA:  Asian-Australas J Anim Sci     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  629-34     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast ...
Next Document:  Induction of Ski Protein Expression upon Luteinization in Rat Granulosa Cells.