| Growth of Mycobacterium leprae in a redox system. | |
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MedLine Citation:
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PMID: 6197589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mycobacteria recovered from human lepromatous nodules and presumably M. leprae, have been grown in a medium that ensured a minimal oxygen tension at initiation of growth, and an increasing availability of oxygen as bacillary growth increased requiring marginal increments in oxygen tension. This physicochemical environment was achieved by the addition of strong biological reductants in the medium, and a combination of partial vacuum and alkaline pyrogallol in the culture vessel. In addition, n-tetradecane, a straight chain hydrocarbon, and lipids like Cholesterol and Lecithin, all three substances mixed in the aqueous medium as liposomes, were added and found to be useful. Menadione, or Vitamin K3, added to the medium considerably improved growth efficiency. Growth occurred initially as non-acid fast coccoids and bacilli that gradually changed to acid-fast bacilli and globi, and cell-wall deficient, spherical L-form elements. Appearance of growth in any form was perceptible within 1 to 2 weeks and optimal growth as acid fast bacilli took upto 3 months. Both the acid fast and non-acid fast bacilli could not be grown in conventional media, but the non-acid fast coccoids could be readily isolated from these cultures in a specially enriched liquid medium. The problem of harvesting of the growth free of lipid-hydrocarbon substances has still not been solved, as also an optimum oxidation-reduction potential. The growth is transferable. |
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Authors:
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B R Chatterjee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leprosy in India Volume: 55 ISSN: 0024-1024 ISO Abbreviation: Lepr India Publication Date: 1983 Jul |
Date Detail:
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Created Date: 1984-02-14 Completed Date: 1984-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376624 Medline TA: Lepr India Country: INDIA |
Other Details:
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Languages: eng Pagination: 426-49 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkanes
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pharmacology Animals Bacteriological Techniques Blood Cholesterol / pharmacology Culture Media* Humans Monophenol Monooxygenase / metabolism Mycobacterium leprae / cytology, growth & development*, metabolism Oxidation-Reduction Oxygen / pharmacology* Penicillin G / pharmacology Phosphatidylcholines / pharmacology Sheep / blood Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 0/Culture Media; 0/Phosphatidylcholines; 57-88-5/Cholesterol; 61-33-6/Penicillin G; 629-59-4/n-tetradecane; 7782-44-7/Oxygen; EC 1.14.18.1/Monophenol Monooxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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