Document Detail


Growth of H-35 rat hepatoma cells in unsupplemented serum-free media: effect of transferrin, insulin and cell density.
MedLine Citation:
PMID:  3284876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serum-free tissue culture medium consisting of a 1:1 mixture of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 medium is herein shown to support growth of Reuber H-35 cells over several days in culture. Cells were initially plated in serum containing DMEM medium for 3 h. After cell attachment, serum is removed and replaced with a serum-free 1:1 mixture of these two commercially available tissue culture media. The doubling time of cell growth in this unsupplemented serum-free medium was 46 h in lightly plated cultures over the first 5 d. The presence of transferrin (5 micrograms/ml) and insulin (3.3 nM) results in a cell doubling time of 17 h, which equaled the growth rate in medium containing 10% fetal bovine serum. In the absence of transferrin, growth rates in serum-free medium were correlated with the cell density of cultures. Conditioned medium from dense, serum-free cultures has growth-stimulating activity in recipient lightly plated cultures. This simple, serum-free culture medium will facilitate studies on the growth regulation of H-35 rat hepatoma cells.
Authors:
L E Shapiro; N Wagner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  24     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-06-14     Completed Date:  1988-06-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  299-303     Citation Subset:  IM    
Affiliation:
Department of Medicine, Montefiore Medical Center, Bronx, New York.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division / drug effects
Culture Media
Growth Substances
Insulin / pharmacology*
Liver Neoplasms, Experimental / pathology*
Rats
Transferrin / pharmacology*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA 16463-14/CA/NCI NIH HHS; CA 24604-09/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Growth Substances; 11061-68-0/Insulin; 11096-37-0/Transferrin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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