Document Detail

Growth of Desulfovibrio on the surface of agar media.
MedLine Citation:
PMID:  5955798     Owner:  NLM     Status:  MEDLINE    
Growth of Desulfovibrio desulfuricans (API strain) was found to take place in an atmosphere of hydrogen on the agar surface of complex media, including yeast extract (Difco), and Trypticase Soy Agar (BBL) without any added reducing agents. For growth on a 2% yeast extract-agar surface in the absence of hydrogen (nitrogen atmosphere), sodium lactate was required in the medium. Growth on the surface of Trypticase Soy Agar (TSA) under nitrogen took place readily in the absence of an added hydrogen donor. A medium (TSA plus salts) is described based upon the addition of sodium lactate (4 ml per liter), magnesium sulfate (2 g per liter), and ferrous ammonium sulfate (0.05%) to TSA, which appears suitable for the isolation and growth of Desulfovibrio on the surface of agar plates in an atmosphere of hydrogen. Sodium lactate does not appear to be essential in this medium for good growth and sulfate reduction in a hydrogen atmosphere, but is essential in a nitrogen atmosphere. Growth of Desulfovibrio (hydrogen atmosphere) on the agar surface of media commonly used for its cultivation as well as on an inorganic medium containing bicarbonate as a source of carbon is poor and erratic unless inoculated (Desulfovibrio) plates of TSA plus salts are incubated in the same container with plates of these media. This stimulatory effect of incubation with inoculated plates of TSA plus salts medium appears to be due to as yet unidentified volatile material produced by D. desulfuricans when growing on this medium. Another volatile material, or possibly the identical material, appears to act similarly to a hydrogen donor.
W P Iverson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied microbiology     Volume:  14     ISSN:  0003-6919     ISO Abbreviation:  Appl Microbiol     Publication Date:  1966 Jul 
Date Detail:
Created Date:  1967-03-10     Completed Date:  1967-03-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605802     Medline TA:  Appl Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  529-34     Citation Subset:  IM    
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MeSH Terms
Acetates / pharmacology
Bicarbonates / pharmacology
Culture Media
Desulfovibrio / growth & development*
Iron / pharmacology
Magnesium / pharmacology
Sulfates / metabolism
Reg. No./Substance:
0/Acetates; 0/Bicarbonates; 0/Culture Media; 0/Sulfates; 1333-74-0/Hydrogen; 7439-89-6/Iron; 7439-95-4/Magnesium; 7727-37-9/Nitrogen; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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