Document Detail


Growth of Actinobacillus pleuropneumoniae is promoted by exogenous hydroxamate and catechol siderophores.
MedLine Citation:
PMID:  8975614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Siderophores bind ferric ions and are involved in receptor-specific iron transport into bacteria. Six types of siderophores were tested against strains representing the 12 different serotypes of Actinobacillus pleuropneumoniae. Ferrichrome and bis-catechol-based siderophores showed strong growth-promoting activities for A. pleuropneumoniae in a disk diffusion assay. Most strains of A. pleuropneumoniae tested were able to use ferrichrome (21 of 22 or 95%), ferrichrome A (20 of 22 or 90%), and lysine-based bis-catechol (20 of 22 or 90%), while growth of 36% (8 of 22) was promoted by a synthetic hydroxamate, N5-acetyl-N5-hydroxy-L-ornithine tripeptide. A. pleuropneumoniae serotype 1 (strain FMV 87-682) and serotype 5 (strain 2245) exhibited a distinct yellow halo around colonies on Chrome Azurol S agar plates, suggesting that both strains can produce an iron chelator (siderophore) in response to iron stress. The siderophore was found to be neither a phenolate nor a hydroxamate by the chemical tests of Arnow and Csaky, respectively. This is the first report demonstrating the production of an iron chelator and the use of exogenous siderophores by A. pleuropneumoniae. A spermidine-based bis-catechol siderophore conjugated to a carbacephalosporin was shown to inhibit growth of A. pleuropneumoniae. A siderophore-antibiotic-resistant strain was isolated and shown to have lost the ability to use ferrichrome, synthetic hydroxamate, or catechol-based siderophores when grown under conditions of iron restriction. This observation indicated that a common iron uptake pathway, or a common intermediate, for hydroxamate- and catechol-based siderophores may exist in A. pleuropneumoniae.
Authors:
M S Diarra; J A Dolence; E K Dolence; I Darwish; M J Miller; F Malouin; M Jacques
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-12-31     Completed Date:  1996-12-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  853-9     Citation Subset:  IM    
Affiliation:
Departement de Microbiologie, Faculte de Medecine, Universite Laval, Sainte-Foy, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Actinobacillus pleuropneumoniae / drug effects,  growth & development*,  metabolism
Anti-Bacterial Agents / pharmacology
Bacterial Outer Membrane Proteins / metabolism
Catechols / pharmacology*
Hydroxamic Acids / pharmacology*
Immunoblotting
Siderophores / pharmacology*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Bacterial Outer Membrane Proteins; 0/Catechols; 0/Hydroxamic Acids; 0/Siderophores
Comments/Corrections

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