Document Detail


Group I mGluR ligands fail to affect 6-hydroxydopamine- induced death and glutamate release of PC12 cells.
MedLine Citation:
PMID:  12852828     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To study the effect of group I metabotropic glutamate receptor (mGluR) ligands on 6-hydroxydopamine (6-OHDA)-induced death and glutamate release of PC12 cells. METHODS: PC12 cells were exposed to 100 micromol/L of group I mGluR agonist (RS)-3,5-dihydroxy-phenylglycine (DHPG) or antagonist DL-2-amino-3-phosphonopropionic acid (DL-AP3) 1 h before addition of 6-OHDA 100 micromol/L. After incubation for 24 h, morphological alterations were observed with microscope, DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method, cytotoxicity was measured by MTT assay, and glutamate release was assayed by high performance liquid chromatography. RESULTS: 6-OHDA decreased cell viability (P<0.01) and induced concentration- and time-dependent glutamate release from PC12 cells. Group I mGluR ligands did not affect 6-OHDA-induced death of PC12 cells and had no influence on glutamate levels. CONCLUSION: Group I mGluR ligands cannot protect PC12 cells from 6-OHDA-induced death.
Authors:
Hao Li; Jian-Hua Ding; Gang Hu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  24     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-07-10     Completed Date:  2004-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  641-5     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Neurobiology, Nanjing Medical University, Nanjing 210029, China.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic Agents / pharmacology
Alanine / analogs & derivatives*,  pharmacology
Animals
Apoptosis*
Cell Survival / drug effects
DNA Fragmentation
Drug Interactions
Glutamic Acid / metabolism*
Ligands
Methoxyhydroxyphenylglycol / analogs & derivatives*,  pharmacology*
Oxidopamine / pharmacology*
PC12 Cells
Rats
Receptors, Metabotropic Glutamate / agonists*,  antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Adrenergic Agents; 0/Ligands; 0/Receptors, Metabotropic Glutamate; 1199-18-4/Oxidopamine; 3343-19-9/dihydroxyphenylethylene glycol; 534-82-7/Methoxyhydroxyphenylglycol; 56-41-7/Alanine; 56-86-0/Glutamic Acid; 5652-28-8/2-amino-3-phosphonopropionic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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