Document Detail

Ground state depletion microscopy for imaging interactions between gold nanowires and fluorophore-labeled ligands.
MedLine Citation:
PMID:  23138571     Owner:  NLM     Status:  Publisher    
Ground state depletion with individual molecule return (GSDIM) is used to interrogate the location of individual fluorescence bursts from fluorophore-labelled DNA molecules on gold nanowire surfaces. Carboxytetramethyl rhodamine (TAMRA)-labelled double-stranded DNA molecules were bound to the surface of gold nanowires via gold-thiol linkages. Individual fluorescence bursts were spatially localized using point spread function fitting and used to reconstruct the image of the underlying nanowire. While the reconstructed images reproduce the size and shape of the nanowire structures, plasmonic coupling between the nanowire and fluorophore is observed, indicating that the location of the observed fluorescence may not precisely correlate with the location of the emitting fluorophore. Thus, plasmonic coupling is an important factor when using super-resolution imaging techniques to study plasmonic nanostructures.
Karole L Blythe; Kathryn M Mayer; Maggie L Weber; Katherine A Willets
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  Physical chemistry chemical physics : PCCP     Volume:  -     ISSN:  1463-9084     ISO Abbreviation:  Phys Chem Chem Phys     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888160     Medline TA:  Phys Chem Chem Phys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Chemistry and Biochemistry, The University of Texas at Austin, Welch Hall 2.204, 105 E. 24th St. STOP A5300, Austin, Texas 78712-1224, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Metabolic preconditioning of mammalian cells: mimetic agents for hypoxia lack fidelity in promoting ...
Next Document:  Sensitivities of rat primary sensory afferent nerves to magnesium: implications for differential ner...