Document Detail


Ground-state transcriptional requirements for skin-derived precursors.
MedLine Citation:
PMID:  23316968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Skin-derived precursors (SKPs) are an attractive stem cell model for cell-based therapies. SKPs can be readily generated from embryonic and adult mice and adult humans, exhibit a high degree of multipotency, and have the potential to serve as a patient autologous stem cell. The advancement of these cells toward therapeutic use depends on the ability to control precisely the self-renewal and differentiation of SKPs. Here we show that two well-known stem cell factors, Foxd3 and Sox2, are critical regulators of the stem cell properties of SKPs. Deletion of Foxd3 completely abolishes the sphere-forming potential of these cells. In the absence of Sox2, SKP spheres can be formed, but with reduced size and frequency. Our results provide entry points into the gene regulatory networks dictating SKP behavior, and pave the way for future studies on a therapeutically relevant stem cell.
Authors:
Michael T Suflita; Elise R Pfaltzgraff; Nathan A Mundell; Larysa H Pevny; Patricia A Labosky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-02-27
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-05-30     Completed Date:  2013-12-17     Revised Date:  2014-04-21    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1779-88     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Embryo, Mammalian
Embryonic Stem Cells / cytology,  metabolism*
Forkhead Transcription Factors / deficiency,  genetics*
Gene Expression Regulation, Developmental*
Mice
Mice, Transgenic
Multipotent Stem Cells / cytology,  metabolism*
Primary Cell Culture
Repressor Proteins / deficiency,  genetics*
SOXB1 Transcription Factors / deficiency,  genetics*
Signal Transduction
Skin / cytology,  embryology,  metabolism*
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
5P01GM085354/GM/NIGMS NIH HHS; HD036720-11S109/HD/NICHD NIH HHS; HD36720/HD/NICHD NIH HHS; NS065604/NS/NINDS NIH HHS; T32 GM007628/GM/NIGMS NIH HHS; T32HD007043/HD/NICHD NIH HHS; T32HD007502/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Forkhead Transcription Factors; 0/Foxd3 protein, mouse; 0/Repressor Proteins; 0/SOXB1 Transcription Factors; 0/Sox2 protein, mouse

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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