Document Detail


GroEL provides protection against Bacillus anthracis infection in BALB/c mice.
MedLine Citation:
PMID:  20832865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heat shock proteins (Hsps) of the HSP60 and HSP70 family are highly conserved and essential to all living organisms. Hsps are immunodominant in numerous microbial infections and have been investigated for their vaccine potential. We investigated the immunogenicity and protective efficacy of GroEL and DnaK of B. anthracis in murine model. Both Hsps were found to be highly immunogenic with mixed antibody response (both IgG1 and IgG2a), indicating stimulation of both humoral and cell-mediated immunity. Cytokine profile also confirmed robust T-cell response with increase in lymphocyte proliferation. Immunization with GroEL conferred 100% protection to mice against B. anthracis infection whereas DnaK couldn't provide protection.
Authors:
Kanchan Sinha; Rakesh Bhatnagar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-15
Journal Detail:
Title:  Molecular immunology     Volume:  48     ISSN:  1872-9142     ISO Abbreviation:  Mol. Immunol.     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2010-11-29     Completed Date:  2011-01-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  264-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2010. Published by Elsevier Ltd.
Affiliation:
School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India. kanchansinha2204@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / immunology*
Animals
Anthrax / immunology*
Antigens, Bacterial / immunology*
Bacillus anthracis / immunology*
Bacterial Proteins / immunology*
Cell Separation
Chaperonin 60 / immunology*
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Lymphocyte Activation / immunology
Mice
Mice, Inbred BALB C
Molecular Chaperones / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Chaperonin 60; 0/Molecular Chaperones; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/DnaK protein, Brevibacillus choshinensis

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