Document Detail

Griseofulvin enhances the effect of aminolevulinic acid-based photodynamic therapy in vitro.
MedLine Citation:
PMID:  16706697     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: In this study, we investigated whether or not griseofulvin (GF), which is an antimycotic widely used for the oral treatment of skin fungal infections, enhanced the effect of aminolevulinic acid-based photodynamic therapy (ALA-PDT) in vitro, using several tumor cell lines. METHODS: A human squamous cell carcinoma line (KB), two human osteosarcoma cell lines from mandible (HOSM-1, HOSM-2), and the human gingiva-derived fibroblast line (HF), representing normal cells, were used. GF enhancement of ALA-PDT was evaluated by comparing the effect of ALAin combination with GF to the effect of ALAalone (GF enhancement rate of ALA-PDT). Also, the effect of GF on intracellular accumulation of protoporphyrin IX (PpIX) was evaluated by comparing the intracellular accumulation of PpIX in the ALA and GF combined treatment with that of ALA treatment alone (pGF enhancement rate of intracellular PpIX). RESULTS: GFenhancement rate of ALA-PDT was 2.51 in KB cells, and 1.65 and 1.27 in HOSM-1 and HOSM-2 cells, respectively. GF enhancement rates of intracellular PpIX were 1.94 in KB cells, 1.53 in HOSM-1 cells, and 1.19 in HOSM-2 cells. GF enhancement rate of intracellular PpIX followed the same trends as the levels of GF enhancement rate of ALA-PDT in the different cell types. For HF, a large effect was not revealed in this study. CONCLUSION: The present study, although preliminary, strongly suggests that concomitant treatment with ALAand GF may be very useful to enhance the effect of ALA-PDT.
Jouji Nomura; Shigeaki Yanase; Tohru Tokuda; Yoshihiko Matsumura; Motoko Sekida; Toshiro Tagawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  24     ISSN:  1549-5418     ISO Abbreviation:  -     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  186-91     Citation Subset:  IM    
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Japan.
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MeSH Terms
Aminolevulinic Acid / pharmacology*
Antifungal Agents / pharmacology*
Griseofulvin / pharmacology*
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology
Protoporphyrins / analysis
Tumor Cells, Cultured
Reg. No./Substance:
0/Antifungal Agents; 0/Photosensitizing Agents; 0/Protoporphyrins; 106-60-5/Aminolevulinic Acid; 126-07-8/Griseofulvin; 553-12-8/protoporphyrin IX

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