Document Detail


Green tea polyphenol inhibits Mycobacterium tuberculosis survival within human macrophages.
MedLine Citation:
PMID:  16352457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lack of maturation of phagosomes containing pathogenic Mycobacterium tuberculosis within macrophages has been widely recognized as a crucial factor for the persistence of mycobacterial pathogen. Host molecule tryptophan-aspartate containing coat protein (TACO) has been shown to play a crucial role in the arrest of such a maturation process. The present study was addressed to understand whether or not polyphenols derived from green tea could down-regulate TACO gene transcription. And if yes, what impact TACO gene down-regulation has on the uptake/survival of M. tuberculosis within macrophages. The reverse-transcriptase polymerase chain reaction and reporter assay technology, employed in this study, revealed that the major component of green tea polyphenols, epigallocatechin-3-gallate had the inherent capacity to down-regulate TACO gene transcription within human macrophages through its ability to inhibit Sp1 transcription factor. We also found out that TACO gene promoter does contain Sp1 binding sequence using bioinformatics tools. The down-regulation of TACO gene expression by epigallocatechin-3-gallate was accompanied by inhibition of mycobacterium survival within macrophages as assessed through flow cytometry and colony counts. Based on these results, we propose that epigallocatechin-3-gallate may be of importance in the prevention of tuberculosis infection.
Authors:
Paras K Anand; Deepak Kaul; Meera Sharma
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The international journal of biochemistry & cell biology     Volume:  38     ISSN:  1357-2725     ISO Abbreviation:  Int. J. Biochem. Cell Biol.     Publication Date:  2006  
Date Detail:
Created Date:  2006-01-25     Completed Date:  2006-06-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9508482     Medline TA:  Int J Biochem Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  600-9     Citation Subset:  IM    
Affiliation:
Molecular Biology Unit, Department of Experimental Medicine and Biotechnology, Chandigarh 160 012, India.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / chemistry,  pharmacology*,  therapeutic use
Camellia sinensis* / chemistry
Catechin / analogs & derivatives*,  chemistry,  pharmacology,  therapeutic use
Colony Count, Microbial
Down-Regulation / drug effects
Flavonoids / chemistry,  pharmacology
Flow Cytometry
Humans
Jurkat Cells
Macrophages / metabolism*,  microbiology
Microfilament Proteins / biosynthesis
Mycobacterium tuberculosis / growth & development*
Phenols / chemistry,  pharmacology
Plant Extracts / chemistry,  pharmacology*,  therapeutic use
Sp1 Transcription Factor / antagonists & inhibitors,  metabolism
Tuberculosis / prevention & control
Grant Support
ID/Acronym/Agency:
N01 AI-75320/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Flavonoids; 0/Microfilament Proteins; 0/Phenols; 0/Plant Extracts; 0/Sp1 Transcription Factor; 0/polyphenols; 145420-64-0/coronin proteins; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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