Document Detail

Green tea polyphenol (-)-epigallocatechin-3-gallate induces neurorescue of long-term serum-deprived PC12 cells and promotes neurite outgrowth.
MedLine Citation:
PMID:  15934936     Owner:  NLM     Status:  MEDLINE    
Our previous studies have shown that the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) prevents neuronal cell death caused by several neurotoxins. The present study sought to determine the neuroprotective effect of EGCG when it is administered after the induction of cell damage ('neurorescue'). In an attempt to imitate a progressive mode of death, PC12 cells were initially subjected to serum-starvation conditions for a period of 1 or 3 days before administration of EGCG (0.1-10 microM) for up to 3 days. In spite of the high percentage of cell death, single or repetitive administration of EGCG (1 microM) significantly attenuated cell death. The neurorescue effect of EGCG was abolished by pre-treatment with the protein kinase C inhibitor GF109203X (2.5 microM), suggesting the involvement of the protein kinase C pathway in neurorescue by the drug. This is consistent with the rapid (15 min) translocation of the protein kinase C alpha isoform to the cell membrane in response to EGCG. The correlative neurite outgrowth activity of EGCG on PC12 cells may also contribute to its neurorescue effect. The present findings suggest that EGCG may have a positive impact on aging and neurodegenerative diseases to retard or perhaps even reverse the accelerated rate of neuronal degeneration.
L Reznichenko; T Amit; M B H Youdim; S Mandel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  93     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-06     Completed Date:  2005-06-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1157-67     Citation Subset:  IM    
Eve Topf and NPF Centers of Excellence for Neurodegenerative Diseases Research and Department of Pharmacology, Technion-Rappaport Family Faculty of Medicine, Haifa, Israel.
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MeSH Terms
Biological Transport / drug effects
Catechin / analogs & derivatives*,  pharmacology*
Cell Death / drug effects
Cell Differentiation / drug effects
Cell Membrane / enzymology
Culture Media, Serum-Free / pharmacology*
Enzyme Activation / drug effects
Flavonoids / pharmacology*
Neurites / drug effects,  physiology*
Neuroprotective Agents / pharmacology*
PC12 Cells / drug effects*,  enzymology,  pathology,  physiology
Phenols / pharmacology*
Protein Kinase C / metabolism,  physiology
Protein Kinase C-alpha
Reg. No./Substance:
0/Culture Media, Serum-Free; 0/Flavonoids; 0/Neuroprotective Agents; 0/Phenols; 0/polyphenols; 154-23-4/Catechin; 989-51-5/epigallocatechin gallate; EC Kinase C; EC Kinase C-alpha

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