Document Detail


Green tea extract selectively targets nanomechanics of live metastatic cancer cells.
MedLine Citation:
PMID:  21451222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Green tea extract (GTE) is known to be a potential anticancer agent (Yang et al 2009 Nat. Rev. Cancer 9 429-39) with various biological activities (Lu et al 2005 Clin. Cancer Res. 11 1675-83; Yang et al 1998 Carcinogenesis 19 611-6) yet the precise mechanism of action is still unclear. The biomechanical response of GTE treated cells taken directly from patient's body samples was measured using atomic force microscopy (AFM) (Binnig et al 1986 Phys. Rev. Lett. 56 930). We found significant increase in stiffness of GTE treated metastatic tumor cells, with a resulting value similar to untreated normal mesothelial cells, whereas mesothelial cell stiffness after GTE treatment is unchanged. Immunofluorescence analysis showed an increase in cytoskeletal-F-actin in GTE treated tumor cells, suggesting GTE treated tumor cells display mechanical, structural and morphological features similar to normal cells, which appears to be mediated by annexin-I expression, as determined by siRNA analysis of an in vitro cell line model. Our data indicates that GTE selectively targets human metastatic cancer cells but not normal mesothelial cells, a finding that is significantly advantageous compared to conventional chemotherapy agents.
Authors:
Sarah E Cross; Yu-Sheng Jin; Qing-Yi Lu; Jianyu Rao; James K Gimzewski
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-30
Journal Detail:
Title:  Nanotechnology     Volume:  22     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-03-31     Completed Date:  2011-07-25     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  215101     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Cell Line, Tumor
Elastic Modulus / drug effects
Female
Humans
Male
Middle Aged
Neoplasm Metastasis / drug therapy
Neoplasms / drug therapy*
Phytotherapy*
Plant Extracts / therapeutic use*
Tea / metabolism*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
1R03CA125859/CA/NCI NIH HHS; 5 R21 GM074509/GM/NIGMS NIH HHS; R03 CA125859-01A1/CA/NCI NIH HHS; R21 GM074509-01/GM/NIGMS NIH HHS; U01CA96116/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Plant Extracts; 0/Tea
Comments/Corrections

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