Document Detail


Greater ATP dependence than sodium dependence of radiocalcium efflux in bullfrog ventricle.
MedLine Citation:
PMID:  3874556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
45Ca efflux was studied in intact bullfrog ventricles following a 2-h period of loading with radiocalcium-containing Ringer solution. The cannulated ventricle was placed in a closed air-filled container to which were applied rhythmic, electronically timed, positive- and negative-pressure pulsations, which induced ventricular volume excursions. The mechanical arrangement and timing circuitry made it possible for each period to be as short in duration as 15 s. By use of this technique, penetration of the extracellular space by [14C]inulin was found to be complete within 30 s, and recovery of the inulin proceeded with a time constant of 17-24 s, indicating a completeness of recovery of 98% within 90 s. Washout of added 45Ca was quantitatively quite close to that of inulin, and in addition the estimated rate of sequestration of the isotope was slow enough to introduce only a small error into the experimental results. 45Ca efflux was only slightly (15%) sensitive to replacement of extracellular sodium but was profoundly sensitive to the inhibitors of ATP synthesis, cyanide and 2-deoxy-glucose.
Authors:
G Brommundt; F Kavaler
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  249     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1985 Jul 
Date Detail:
Created Date:  1985-08-12     Completed Date:  1985-08-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C129-39     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / physiology*
Animals
Biological Transport, Active / drug effects
Calcium / metabolism*
Calcium Radioisotopes / diagnostic use
Deoxyglucose / pharmacology
Extracellular Space / metabolism
Heart Ventricles / anatomy & histology,  metabolism
Inulin / diagnostic use
Kinetics
Myocardium / metabolism*
Rana catesbeiana
Sodium / physiology*
Sodium Cyanide / pharmacology
Temperature
Chemical
Reg. No./Substance:
0/Calcium Radioisotopes; 143-33-9/Sodium Cyanide; 154-17-6/Deoxyglucose; 56-65-5/Adenosine Triphosphate; 7440-23-5/Sodium; 7440-70-2/Calcium; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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