Document Detail


Gratuitous mRNA localization in the Drosophila oocyte.
MedLine Citation:
PMID:  7555727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many of the genes that control pattern formation in Drosophila encode mRNAs that are localized to discrete regions of the oocyte during oogenesis. While such localization is generally assumed to be important for the pattern-forming activities of these genes, this has been rigorously demonstrated in only a few cases. Here we address the role of mRNA localization for the dorsoventral patterning gene K10. K10 mRNA is localized to the oocyte's anterior cortex following its transport into the cell during early stages of oogenesis. We show that mutations in cappuccino and spire, which permit K10 mRNA transport, but prevent subsequent anterior localization, do not disrupt the synthesis or localization of K10 protein. We also show that modified K10 transgenes that produce transcripts which are uniformly distributed throughout the oocyte, or which are mislocalized to the oocyte's posterior pole, produce localized and functional K10 protein. We conclude that the anterior localization of K10 mRNA is not important for K10 protein targeting or gene function. We propose that the anterior localization of K10, and probably other mRNAs, is a by-product of mRNA transport and does not necessarily reflect a requirement for localization per se.
Authors:
T L Serano; R S Cohen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  121     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-08     Completed Date:  1995-11-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3013-21     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Kansas, Lawrence 66045, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U36587
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MeSH Terms
Descriptor/Qualifier:
Animals
Drosophila / embryology*,  genetics
Drosophila Proteins*
Female
Genes, Insect*
Immunohistochemistry
In Situ Hybridization
Morphogenesis / genetics
Mutation
Nuclear Proteins / genetics*
Oocytes / physiology*
Oogenesis / genetics,  physiology
RNA, Messenger / physiology*
Transcription Factors / genetics*
Transgenes
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Nuclear Proteins; 0/RNA, Messenger; 0/Transcription Factors; 0/fs(1)K10 protein, Drosophila

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