Document Detail


Graphical analysis of flow cytometer data for characterizing controlled fluorescent protein display on λ phage.
MedLine Citation:
PMID:  23027705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
As native virus particles typically cannot be resolved using a flow cytometer, the general practice is to use fluorescent dyes to label the particles. In this work, an attempt was made to use a common commercial flow cytometer to characterize a phage display strategy that allows for controlled levels of protein display, in this case, eGFP. To achieve this characterization, a number of data processing steps were needed to ensure that the observed phenomena were indeed capturing differences in the phages produced. Phage display of eGFP resulted in altered side scatter and fluorescence profile, and sub-populations could be identified within what would otherwise be considered uniform populations. Surprisingly, this study has found that side scatter may be used in the future to characterize the display of nonfluorescent proteins.
Authors:
Stanislav Sokolenko; Jessica Nicastro; Roderick Slavcev; Marc G Aucoin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  81     ISSN:  1552-4930     ISO Abbreviation:  Cytometry A     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-21     Completed Date:  2013-04-25     Revised Date:  2013-07-09    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1031-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 International Society for Advancement of Cytometry.
Affiliation:
Department of Chemical Engineering, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
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MeSH Terms
Descriptor/Qualifier:
Bacteriophage lambda / chemistry*,  genetics,  growth & development
Capsid Proteins / chemistry,  genetics
Cell Surface Display Techniques / methods*
Computer Graphics*
Escherichia coli / chemistry,  virology
Flow Cytometry / methods*
Fluorescence
Green Fluorescent Proteins / chemistry*,  genetics
Microscopy, Fluorescence
Plasmids / chemistry,  genetics
Temperature
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/D protein, Enterobacteria phage lambda; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections
Erratum In:
Cytometry A. 2013 Jun;83(6):592

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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