Document Detail


Graphical analysis of data from pharmacology experiments.
MedLine Citation:
PMID:  1409246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dose-response curves are often used in the study of the interaction of hormones and receptors. From these plots, IC50 or EC50 values are calculated. In these pharmacological assays it is implicitly assumed that a single receptor predominates in a tissue. In this paper the interaction of a ligand with two receptors is studied from a theoretical point of view. It is assumed that the responses mediated by these receptors are qualitatively or quantitatively different. The theoretical direct and Scatchard plots display a high variety of shapes depending upon the difference in potency of the effect of the drug acting in both receptors and upon the magnitude and sign of the individual response. When dose-response curves taken from the literature have been transformed into direct or Scatchard plots new information has become available. With respect to this, it is shown that agonists of purinergic receptors seem to interact with two different populations of receptors. We claim that carefully designed experiments must provide valuable information concerning the number of subtypes of receptors present in a given system and the kind of response mediated by them.
Authors:
J J Centelles; M Busquets; E I Canela; R Franco
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  25     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:    1992 May-Jun
Date Detail:
Created Date:  1992-10-26     Completed Date:  1992-10-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  325-34     Citation Subset:  IM    
Affiliation:
Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Catalunya, Spain.
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MeSH Terms
Descriptor/Qualifier:
Adenosine / metabolism
Adenosine Triphosphate / analogs & derivatives,  metabolism,  pharmacology
Animals
Brain / drug effects,  metabolism
Dose-Response Relationship, Drug*
Duodenum / drug effects
Mathematics
Rabbits
Rats
Receptors, Drug / metabolism*
Receptors, Purinergic / metabolism
Regression Analysis
Vasoconstriction / drug effects
Chemical
Reg. No./Substance:
0/Receptors, Drug; 0/Receptors, Purinergic; 56-65-5/Adenosine Triphosphate; 58-61-7/Adenosine; 7292-42-4/alpha,beta-methyleneadenosine 5'-triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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