Document Detail

Graphene oxide protected nucleic Acid probes for bioanalysis and biomedicine.
MedLine Citation:
PMID:  23839798     Owner:  NLM     Status:  In-Data-Review    
Recently, the binding ability of DNA on GO and resulting nuclease resistance have attracted increasing attention, leading to new applications both in vivo and in vitro. In vivo, nucleic acids absorbed on GO can be effectively protected from enzymatic degradation and biological interference in complicated samples, making it useful for targeted delivery, gene regulation, intracellular detection and imaging with high uptake efficiencies, high intracellular stability, and very low toxicity. In vitro, the adsorption of ssDNA on GO surface and desorption of dsDNA or well-folded ssDNA from GO surface result in the protection and deprotection of DNA from nucleic digestion, respectively, which has led to target-triggered cyclic enzymatic amplification methods (CEAM) for amplified detection of analytes with sensitivity 2-3 orders of magnitude higher than that of 1:1 binding strategies. This Concept article explores some of the latest developments in this field.
Liang Cui; Yanling Song; Guoliang Ke; Zhichao Guan; Huimin Zhang; Ya Lin; Yishun Huang; Zhi Zhu; Chaoyong James Yang
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Publication Detail:
Type:  Journal Article     Date:  2013-07-09
Journal Detail:
Title:  Chemistry (Weinheim an der Bergstrasse, Germany)     Volume:  19     ISSN:  1521-3765     ISO Abbreviation:  Chemistry     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513783     Medline TA:  Chemistry     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  10442-51     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
State Key Laboratory of Physical Chemistry of Solid Surfaces, The Key Laboratory for Chemical Biology of Fujian Province, Key Laboratory of Analytical Science and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005 (China), Tel: (+86) 592-218-7601.
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