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Graphene oxide based ultrasensitive flow-through chemiluminescent immunoassay for sub-picogram level detection of chicken interferon-γ
MedLine Citation:
PMID:  23999207     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The quantitative detection of chicken interferon-γ (ChIFN-γ) released by T cells after in vitro stimulation is a good evaluation of cell-mediated immunity in chickens after infection or vaccination. This work reports a new flow-through the chemiluminescent (CL) immunoassay method for rapid and specific determination of ChIFN-γ at a level of sub-picogram per milliliter. The biocompatible graphene oxide nanosheet is introduced into CL immunoassay for highly efficient immobilization of capture antibody. The detection limit of the proposed method at a signal to noise ratio of 3 is 0.36pgml(-1), which is 138-fold lower than the current lowest value of 50pgml(-1) for ChIFN-γ. Coupling with a flow-through system, the whole immunoassay process can be completed within 25min. The resulting ChIFN-γ immunosensor shows excellent detection and fabrication reproducibility, good specificity and stability. The assay results of nature ChIFN-γ samples with the proposed method are in an acceptable agreement with the reference values. Compared to the present assay methods, this method is more flexible, simple, rapid and sensitive. The aim of this work is to demonstrate that the ultrasensitive, specific and rapid CL immunoassay format can become a very potential application for quantifying ChIFN-γ and further studying its role in immune response in poultry.
Authors:
Zhanjun Yang; Jing Zhu; Hua Dai; Juan Li; Juan Shen; Xinan Jiao; Xiaoya Hu; Huangxian Ju
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-8-12
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  51C     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-9-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  356-361     Citation Subset:  -    
Copyright Information:
© 2013 Elsevier B.V. All rights reserved.
Affiliation:
College of Chemistry and Chemical Engineering; School of Medicine; Jiangsu Key Lab of Zoonosis, Yangzhou University, Yangzhou 225002, PR China. Electronic address: zjyang@yzu.edu.cn.
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