Document Detail


Graphene oxide/poly-l-lysine assembled layer for adhesion and electrochemical impedance detection of leukemia K562 cancer cells.
MedLine Citation:
PMID:  23202339     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel biocompatible film assembled by combining of graphene oxide (GO) and poly-l-lysine (PLL) for adhesion and electrochemical impedance detection of leukemia K562 cells was proposed. The biocompatible film showed an improved immobilization capacity for living cells and a good biocompatibility for preserving the activity of the immobilized living cells. The immobilized K562 cells on the biocompatible film-modified electrode can be directly monitored with electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3-/4-) as redox probes. A highly sensitive electrochemical impedance method for the detection of leukemia K562 cancer cells was developed. Under the optimized conditions, the increased electron-transfer resistance with a good correlation to the logarithmic value of concentration of K562 cells ranging from 10(2) to 10(7)cellsmL(-1), and with the detection limit of 30cellsmL(-1) (S/N=3). Additionally, the proposed method was used to describe the viability of cells and to evaluate the effectiveness of antitumor drug Nilotinib on K562 cells. The obtained results of Nilotinib cytotoxicity are well agreed with those from WST-1 assays. Furthermore, the work demonstrates that a highly biocompatible film of PLL/GO assembled is also expected to be an appropriate matrix for the electrochemical investigation of adhesion, proliferation, apoptosis of other relevant mammalian cells which is not limited to adherent cells, and the study of cell-based biosensors.
Authors:
Dongdong Zhang; Yanmin Zhang; Lei Zheng; Yingzhuan Zhan; Langchong He
Related Documents :
23142219 - Down-regulation of mir-517a and mir-517c promotes proliferation of hepatocellular carci...
24331919 - Inhibition of telomerase induces alternative lengthening of telomeres during human esop...
24206079 - Polymer functionalization as a powerful tool to improve scaffold performances.
23722439 - Inhibitory impacts of chemically modified tetracycline-3 and underlying mechanism in hu...
24148399 - Parallel comparative studies on the toxic effects of unmodified cdte quantum dots, gold...
23719479 - Phlda1, a follicular stem cell marker, differentiates clear-cell/granular-cell trichobl...
23142219 - Down-regulation of mir-517a and mir-517c promotes proliferation of hepatocellular carci...
24331919 - Inhibition of telomerase induces alternative lengthening of telomeres during human esop...
8848279 - Nadph-diaphorase reactivity in the mauthner cells of the swordtail fish, xiphophorus he...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-1
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  42C     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  -    
Other Details:
Languages:  ENG     Pagination:  112-118     Citation Subset:  -    
Copyright Information:
Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.
Affiliation:
School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Simultaneous detection of biomolecular interactions and surface topography using photonic force micr...
Next Document:  Aptamer-based colorimetric detection of platelet-derived growth factor using unmodified gold nanopar...