| Grape seed procyanidin B2 inhibits advanced glycation end product-induced endothelial cell apoptosis through regulating GSK3β phosphorylation. | |
| | |
MedLine Citation:
|
PMID: 21244366 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To investigate the effects of GSPB2 (grape seed procyanidin B2) on the apoptosis of HUVECs (human umbilical endothelial cells) induced by AGEs (advanced glycation end products), HUVECs were treated with AGEs (200 μg/ml) in the presence or absence of GSPB2 (2.5, 5.0 and 10.0 μmol/l). Our findings showed that (i) AGEs induced HUVEC apoptosis and up-regulated the expression of caspase-3 activation and lactadherin and reduced the phosphorylation of GSK3β (glycogen synthase kinase 3β) at baseline. (ii) Treatment of HUVEC with GSPB2 significantly inhibited the cell apoptosis and the expression of caspase-3 activation and lactadherin induced by AGEs. Moreover, GSPB2 inhibited intracellular reactive oxygen species in a dose-dependent manner in AGEs-treated cells as determined by flow cytometry. (iii) GSPB2 increased the phosphorylation of GSK3β of HUVEC in response to AGEs. These findings suggest that the signalling pathway involving phosphorylation of GSK3β and lactadherin might play a key role in the endothelial apoptosis. GSPB2 therapy could become an effective approach to battling AGEs-induced endothelial apoptosis. |
| | |
Authors:
|
Bao-ying Li; Xiao-li Li; Hai-qing Gao; Jian-hua Zhang; Qian Cai; Mei Cheng; Mei Lu |
Related Documents
:
|
21354106 - Overexpression of cellular repressor of e1a-stimulated genes inhibits tnf-α-induced ap... 21422246 - Control of tnf-induced dendritic cell maturation by hybrid-type n-glycans. 21112386 - Tgf-β1 affects endothelial cell interaction with macrophages and t cells leading to th... 15963046 - Cd45ra and ro isoforms have distinct effects on cytokine- and b-cell-receptor-mediated ... 10090946 - Subcellular distribution and redistribution of bcl-2 family proteins in human leukemia ... 19516256 - Cyclic amp intoxication of macrophages by a mycobacterium tuberculosis adenylate cyclase. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell biology international Volume: 35 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-06-20 Completed Date: 2011-10-03 Revised Date: 2011-11-02 |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 663-9 Citation Subset: IM |
Affiliation:
|
Key Laboratory of Cardiovascular Proteomics of Shandong Province, Department of Geriatrics, QiLu Hospital of Shandong University, Jinan, Shandong Province, Peoples Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, Surface
/
genetics,
metabolism* Apoptosis / drug effects* Biflavonoids* / pharmacology, therapeutic use Blotting, Western Caspase 3 / antagonists & inhibitors, genetics, metabolism* Catechin* / pharmacology, therapeutic use Diabetes Mellitus / drug therapy, metabolism, pathology Endothelial Cells / cytology, drug effects* Flow Cytometry Gene Expression / drug effects Glycogen Synthase Kinase 3 / antagonists & inhibitors, genetics, metabolism* Glycosylation End Products, Advanced / adverse effects* Grape Seed Extract / pharmacology, therapeutic use Humans Milk Proteins / antagonists & inhibitors, genetics, metabolism* Oxidative Stress / drug effects Phosphorylation / drug effects Polymerase Chain Reaction Proanthocyanidins* / pharmacology, therapeutic use Reactive Oxygen Species / antagonists & inhibitors*, metabolism Seeds / chemistry Signal Transduction Umbilical Veins / cytology Vitis / chemistry |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Surface; 0/Biflavonoids; 0/Glycosylation End Products, Advanced; 0/Grape Seed Extract; 0/MFGE8 protein, human; 0/Milk Proteins; 0/Proanthocyanidins; 0/Reactive Oxygen Species; 154-23-4/Catechin; 29106-49-8/procyanidin B2; EC 2.7.11.1/glycogen synthase kinase 3 beta; EC 2.7.11.26/Glycogen Synthase Kinase 3; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Endoplasmic Reticulum stress induced by Hepatitis B virus X protein enhances cyclooxygenase 2 expres...
Next Document: Adipogenic differentiation and EGFP gene transfection of amniotic fluid derived stem cells from goat...