Document Detail

Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism.
MedLine Citation:
PMID:  20483185     Owner:  NLM     Status:  MEDLINE    
Staphylococcus aureus (S. aureus) is one of the most common pathogens that causes infectious and foodborne diseases worldwide. Searching for drug and chemical compounds against this bacterium is still in demand. We found that grape seed extract (GSE), a natural food product rich in polyphenols, inhibited the dihydrofolate reductase activity and growth of S. aureus. In addition, the intracellular content of tetrahydrofolate (THF), the major folate species identified in S. aureus, was significantly decreased when GSE was present in medium. The GSE-induced growth inhibition was reversed by adding, THF, 5,10-methylenetetrahydrofolate or methionine to the medium. The differential rescuing effects elicited by thymidine and methionine indicated that GSE-induced perturbation in folate-mediated one-carbon metabolism has more profound impact on methionine cycle than on thymidine monophosphate (TMP) synthesis. Significantly reduced inflammatory responses and mortality were observed in zebrafish infected with S. aureus pre-incubated with GSE. We conclude that GSE might serve as an effective natural alternative for the control of food poisoning caused by S. aureus with proper safety measure.
Tseng-Ting Kao; Hung-Chi Tu; Wen-Ni Chang; Bing-Hung Chen; Ya-Yuang Shi; Tsung-Chain Chang; Tzu-Fun Fu
Related Documents :
19184765 - Validation of the folate food frequency questionnaire with serum and erythrocyte folate...
16464815 - Age and gender effect on the use of herbal medicine products and food supplements among...
10426695 - Estimated folate intakes: data updated to reflect food fortification, increased bioavai...
7288185 - Price of amino acids as a basis for planning protein adequate diets.
8321065 - The role of nutrition in sickness and in health.
21289225 - Hidden vegetables: an effective strategy to reduce energy intake and increase vegetable...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-11
Journal Detail:
Title:  International journal of food microbiology     Volume:  141     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbon / metabolism*
Fish Diseases / drug therapy*,  microbiology
Flavonoids / pharmacology,  therapeutic use
Grape Seed Extract / pharmacology*,  therapeutic use
Methionine / metabolism
Phenols / pharmacology,  therapeutic use
Staphylococcal Food Poisoning / prevention & control
Staphylococcal Infections / drug therapy*,  microbiology
Staphylococcus aureus / drug effects*,  growth & development,  pathogenicity
Tetrahydrofolate Dehydrogenase / metabolism*
Tetrahydrofolates / pharmacology
Thymidine / metabolism
Thymidine Monophosphate / biosynthesis
Vitis / chemistry*
Zebrafish / microbiology
Reg. No./Substance:
0/Flavonoids; 0/Grape Seed Extract; 0/Phenols; 0/Tetrahydrofolates; 0/polyphenols; 29347-89-5/5,6,7,8-tetrahydrofolic acid; 365-07-1/Thymidine Monophosphate; 50-89-5/Thymidine; 63-68-3/Methionine; 7440-44-0/Carbon; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Some growth parameters and antimicrobial activity of a bacteriocin-producing strain Pediococcus acid...
Next Document:  Functional fermented whey-based beverage using lactic acid bacteria.