Document Detail


Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism.
MedLine Citation:
PMID:  20483185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcus aureus (S. aureus) is one of the most common pathogens that causes infectious and foodborne diseases worldwide. Searching for drug and chemical compounds against this bacterium is still in demand. We found that grape seed extract (GSE), a natural food product rich in polyphenols, inhibited the dihydrofolate reductase activity and growth of S. aureus. In addition, the intracellular content of tetrahydrofolate (THF), the major folate species identified in S. aureus, was significantly decreased when GSE was present in medium. The GSE-induced growth inhibition was reversed by adding, THF, 5,10-methylenetetrahydrofolate or methionine to the medium. The differential rescuing effects elicited by thymidine and methionine indicated that GSE-induced perturbation in folate-mediated one-carbon metabolism has more profound impact on methionine cycle than on thymidine monophosphate (TMP) synthesis. Significantly reduced inflammatory responses and mortality were observed in zebrafish infected with S. aureus pre-incubated with GSE. We conclude that GSE might serve as an effective natural alternative for the control of food poisoning caused by S. aureus with proper safety measure.
Authors:
Tseng-Ting Kao; Hung-Chi Tu; Wen-Ni Chang; Bing-Hung Chen; Ya-Yuang Shi; Tsung-Chain Chang; Tzu-Fun Fu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-11
Journal Detail:
Title:  International journal of food microbiology     Volume:  141     ISSN:  1879-3460     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  17-27     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Affiliation:
Institute of Basic Medical Science, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon / metabolism*
Fish Diseases / drug therapy*,  microbiology
Flavonoids / pharmacology,  therapeutic use
Grape Seed Extract / pharmacology*,  therapeutic use
Methionine / metabolism
Phenols / pharmacology,  therapeutic use
Phytotherapy
Staphylococcal Food Poisoning / prevention & control
Staphylococcal Infections / drug therapy*,  microbiology
Staphylococcus aureus / drug effects*,  growth & development,  pathogenicity
Tetrahydrofolate Dehydrogenase / metabolism*
Tetrahydrofolates / pharmacology
Thymidine / metabolism
Thymidine Monophosphate / biosynthesis
Vitis / chemistry*
Zebrafish / microbiology
Chemical
Reg. No./Substance:
0/Flavonoids; 0/Grape Seed Extract; 0/Phenols; 0/Tetrahydrofolates; 0/polyphenols; 29347-89-5/5,6,7,8-tetrahydrofolic acid; 365-07-1/Thymidine Monophosphate; 50-89-5/Thymidine; 63-68-3/Methionine; 7440-44-0/Carbon; EC 1.5.1.3/Tetrahydrofolate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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